Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human APOD 293 Cell Lysate

Cat.No. : APOD-8781HCL
  • Specification
  • Gene Information
  • Related Products
Description : Antigen standard for apolipoprotein D (APOD) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : APOD apolipoprotein D [ Homo sapiens ]
Official Symbol : APOD
Synonyms : APOD; apolipoprotein D; apo-D;
Gene ID : 347
mRNA Refseq : NM_001647
Protein Refseq : NP_001638
MIM : 107740
UniProt ID : P05090
Chromosome Location : 3q26.2-qter
Function : cholesterol binding; lipid binding; lipid transporter activity; protein binding; transporter activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (19)

Ask a question
Are there any known interactions or associations between ApoD protein and other proteins or molecules in the body? 04/05/2023

Yes, ApoD protein has been found to interact with several other proteins and molecules. For example, it has been shown to interact with lipoprotein receptors involved in lipid metabolism, such as LDL receptor-related protein 1 (LRP1). Additionally, ApoD can bind to β-amyloid peptides, which are implicated in Alzheimer's disease. These interactions suggest potential roles for ApoD in modulating lipid transport and neurodegenerative processes.

Are there any genetic disorders associated with mutations in the ApoD gene? 06/29/2021

Mutations in the ApoD gene are not typically associated with any specific genetic disorders. However, variations in the ApoD gene have been linked to certain diseases or conditions, such as Alzheimer's disease, schizophrenia, and Parkinson's disease. The exact mechanisms and significance of these associations are still being investigated.

Can lifestyle factors or medications affect ApoD protein levels? 12/11/2020

Some studies suggest that lifestyle factors and medications may influence ApoD protein levels. For example, certain drugs, such as statins, have been found to increase ApoD protein expression. Additionally, lifestyle factors like exercise and dietary interventions may also impact ApoD levels. However, further research is needed to fully understand the relationship between these factors and ApoD protein.

What are the physiological functions of ApoD protein? 09/30/2020

ApoD protein has diverse physiological functions. It is primarily associated with lipid metabolism and transport, as it binds to lipids and lipophilic molecules, such as fatty acids and cholesterol. ApoD is also involved in stress response mechanisms, including protection against oxidative stress and neuronal damage. It has been implicated in modulating inflammation and immunity as well. Furthermore, ApoD may play a role in promoting neurite outgrowth and neuronal survival.

Is ApoD protein expressed in specific tissues or organs? 09/10/2020

ApoD protein is widely expressed in various tissues and organs throughout the body. It is highly abundant in the central nervous system, including the brain, where it is mainly localized in astrocytes. ApoD expression is also found in other tissues, such as the liver, kidney, adipose tissue, and reproductive organs. Its expression can be regulated by various factors, including hormones, stress, and aging.

Can ApoD protein contribute to neurodegenerative diseases? 07/09/2020

Research suggests that ApoD protein may play a role in neurodegenerative diseases. It has been found to interact with β-amyloid peptides, which form plaques in the brains of individuals with Alzheimer's disease. ApoD can bind to and stabilize β-amyloid, potentially influencing its aggregation and clearance. Additionally, ApoD may have neuroprotective effects by reducing oxidative stress and inflammation. However, the exact contributions of ApoD to neurodegenerative diseases are still being investigated.

What research techniques are used to study the functions and mechanisms of ApoD protein? 12/27/2019

Various techniques are employed to study the functions and mechanisms of ApoD protein. These include in vitro experiments using cell cultures to investigate its interactions, expression, and functions. Animal models, such as knockout or transgenic mice with modified ApoD genes, are also used to study the effects of ApoD protein on specific processes or diseases. Additionally, sophisticated imaging techniques and proteomic analyses can provide insights into the localization and interactions of ApoD within tissues and organs.

Are there any therapeutic implications or potential treatments targeting ApoD protein? 11/20/2019

The potential therapeutic implications of ApoD protein are still being explored. Some studies have suggested that increasing ApoD levels could have neuroprotective effects and may be beneficial in neurodegenerative diseases. Additionally, modulating ApoD expression or function could potentially impact lipid metabolism and associated disorders. However, it is important to note that any potential treatment strategies targeting ApoD are still in the early stages of development and further research is needed.

Is there a link between APOD protein and sleep regulation? 10/14/2019

Yes, studies have shown that APOD levels fluctuate with the sleep-wake cycle, with higher expression during periods of sleep. APOD knockout mice exhibit altered sleep patterns, suggesting a role in the regulation of sleep duration and quality.

Are there any diseases associated with APOD protein? 06/03/2019

APOD has been implicated in various diseases, including cancer, neurodegenerative disorders, obesity, and metabolic disorders. However, further research is needed to fully understand its involvement in these conditions.

Can APOD protein be a therapeutic target for any diseases? 08/02/2018

The potential of APOD protein as a therapeutic target is still being investigated. Its involvement in various diseases suggests that modulating its expression or activity could have therapeutic benefits. However, more research is needed to fully understand its mechanisms and determine its suitability as a target.

How does APOD protein contribute to neuroprotection? 02/26/2018

APOD has been shown to have neuroprotective properties. It may protect against oxidative stress and inflammation, and contribute to the maintenance and survival of neurons. It has been implicated in neurodegenerative disorders like Alzheimer's disease.

Where is ApoD protein found in the body? 10/30/2017

ApoD protein is found in various tissues throughout the body, including the brain, liver, kidneys, and adipose tissue. It is also present in the cerebrospinal fluid and can be detected in plasma.

How is APOD protein involved in lipid metabolism? 10/14/2017

APOD is thought to play a role in the regulation of lipid metabolism and transport. It has been found to interact with lipids and lipoproteins and may contribute to the metabolism and transport of fatty acids and cholesterol.

Can ApoD protein be used as a diagnostic marker for certain diseases? 08/02/2017

ApoD protein has shown potential as a diagnostic marker for certain diseases. For example, its altered levels have been observed in neurodegenerative disorders like Alzheimer's disease and Parkinson's disease. Additionally, changes in ApoD levels have been associated with certain types of cancer, such as breast cancer. However, more research is necessary to establish the reliability and specificity of ApoD as a diagnostic marker.

Are there any ongoing research studies or clinical trials related to ApoD protein? 11/06/2016

Research studies and clinical trials related to ApoD protein are ongoing to further understand its functions and potential implications in various diseases. Some areas of research include investigating its role in neurodegenerative disorders, exploring its potential as a diagnostic marker, and studying its therapeutic potential. Clinical trials may also be exploring interventions targeting ApoD, although specific trials may vary depending on current advancements in the field.

How is ApoD protein measured in laboratory tests or clinical settings? 09/16/2016

ApoD protein levels can be measured in laboratory tests or clinical settings using various techniques. One common method is enzyme-linked immunosorbent assay (ELISA), where antibodies specific to ApoD are used to detect and quantify its concentration in biological samples, such as blood or cerebrospinal fluid.

Is ApoD protein involved in aging-related processes? 06/01/2016

There is evidence suggesting a potential involvement of ApoD protein in aging-related processes. Studies have shown that ApoD levels increase with age in various tissues, including the brain. Additionally, ApoD has been implicated in stress response mechanisms and protecting against oxidative damage, which are both important factors in aging. However, further research is needed to fully understand the specific contributions of ApoD to the aging process.

Does the expression of ApoD protein change with aging? 05/29/2016

Yes, there is evidence that the expression of ApoD protein changes with aging. Studies have shown that ApoD levels increase with age in various tissues, including the brain. This upregulation of ApoD expression may be a protective response to counteract age-related oxidative stress and inflammation. The increased expression of ApoD in older individuals suggests it may have a role in the aging process, but further research is needed to fully understand its implications.

Customer Reviews (4)

Write a review
Reviews
05/31/2022

    Its integrity and reliability make it an ideal choice to address the specific objectives of my research.

    04/15/2022

      Their comprehensive certificates of analysis and safety data sheets serve as testaments to their commitment to ensuring the safety and integrity of their products.

      05/05/2020

        In addition to the exceptional quality of the protein, the manufacturer's support extends beyond the product itself.

        01/31/2016

          Their ability to accommodate the requirements of large-scale experiments and guarantee a steady supply streamlines my research operations, eliminating any concerns related to prospective shortages.

          Ask a Question for All APOD Products

          Required fields are marked with *

          My Review for All APOD Products

          Required fields are marked with *

          0

          Inquiry Basket

          cartIcon
          logo

          FOLLOW US

          Terms and Conditions        Privacy Policy

          Copyright © 2024 Creative BioMart. All Rights Reserved.

          Contact Us

          • /

          Stay Updated on the Latest Bioscience Trends