Recombinant Human AKAP7 293 Cell Lysate

• Cat.No.: AKAP7-8937HCL

Specification

• Description: Antigen standard for A kinase (PRKA) anchor protein 7 (AKAP7), transcript variant beta is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AKAP7 A kinase (PRKA) anchor protein 7 [ Homo sapiens ]
• Official Symbol: AKAP7
• Synonyms: AKAP7; A kinase (PRKA) anchor protein 7; A-kinase anchor protein 7 isoform gamma; AKAP15; AKAP18; AKAP 18; PRKA7 isoform gamma; AKAP-7 isoform gamma; PRKA7 isoforms alpha/beta; A-kinase anchor protein 9 kDa; A-kinase anchor protein 18 kDa; AKAP-7 isoforms alpha and beta; A-kinase anchor protein 7 isoforms alpha and beta
• Gene ID: 9465
• mRNA Refseq: NM_138633
• Protein Refseq: NP_619539
• MIM: 604693
• UniProt ID: O43687
• Chromosome Location: 6q22-q24
• Pathway: G Protein Signaling Pathways, organism-specific biosystem
• Function: AMP binding; catalytic activity; protein C-terminus binding; protein complex scaffold; protein domain specific binding; protein kinase A binding; protein kinase A binding; protein kinase A regulatory subunit binding; protein kinase binding