"ADAMTS4" Related Products

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Recombinant Human ADAMTS4 293 Cell Lysate

Cat.No.: ADAMTS4-9029HCL
Description: Antigen standard for ADAM metallopeptidase with thrombospondin type 1 motif, 4 (ADAMTS4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ADAMTS4 ADAM metallopeptidase with thrombospondin type 1 motif, 4 [ Homo sapiens ]
Official Symbol: ADAMTS4
Synonyms: ADAMTS4; ADAM metallopeptidase with thrombospondin type 1 motif, 4; a disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4; A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAMTS 2; ADMP 1; KIAA0688; ADAM-TS4; ADAM-TS 4; aggrecanase-1; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4; ADMP-1; ADAMTS-2; ADAMTS-4;
Gene ID: 9507
mRNA Refseq: NM_005099
Protein Refseq: NP_005090
MIM: 603876
UniProt ID: O75173
Chromosome Location: 1q31-q32
Pathway: Endochondral Ossification, organism-specific biosystem;
Function: metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; protease binding; protein binding; zinc ion binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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