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Recombinant Human Alanyl-tRNA Synthetase, His-tagged

Cat.No. : AARS-51H
Product Overview : Recombinant HumanAlanyl-tRNA synthetase produced inSf9is a glycosylated, polypeptide chain having a molecular mass of 107,598 Dalton. PL-12 is expressed with a -6x His tag and purified by proprietary chromatographic techniques.
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Cat. No. : AARS-51H
Description : Alanyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 108 kDa protein is an autoantigen recognized by PL-12 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that epitope spreading occurs in the autoimmune PL-12 response such that even antibodies to an isolated alanyl-tRNA molecule can develop.
Source : Sf9 insect cells.
Physical Appearance : Sterile Filtered clear solution.
Purity : Greater than 90% as determined by SDS-PAGE.
Formulation : PL-12 is supplied at a of 0.9 mg/ml in16 mM HEPES buffer pH-8, 250 mM sodium chloride, and 20% glycerol.
Immunological Functions : 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).
Applications : Western-Blot with monoclonal anti-hexa-His-tag antibody & Polymyositis sera.
Coating Concentration : 0.3-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Storage : Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Publication :
Evaluation of paraneoplastic antigens reveals TRIM21 autoantibodies as biomarker for early detection of ovarian cancer in combination with autoantibodies to NY-ESO-1 and TP53 (2020)
Gene Name : AARS alanyl-tRNA synthetase [ Homo sapiens ]
Synonyms : AARS; alanyl-tRNA synthetase; alanine tRNA ligase 1, cytoplasmic; Alanyl-tRNA synthetase, cytoplasmic; Renal carcinoma antigen NY-REN-42; Alanine--tRNA ligase; AlaRS; EC 6.1.1.7
Gene ID : 16
mRNA Refseq : NM_001605
Protein Refseq : NP_001596
MIM : 601065
UniProt ID : P49588
Chromosome Location : 16q22
Pathway : Aminoacyl-tRNA biosynthesis; Gene Expression
Function : ATP binding; alanine-tRNA ligase activity; ligase activity; nucleic acid binding; tRNA binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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