Recombinant Human PLA2G1B protein, His-tagged

• Cat.No.: PLA2G1B-171H
• Product Overview: Recombinant Human PLA2G1B (Ala23-Ser148) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Phosphatidylcholine 2-acylhydrolase 1B (PLA2G1B) is also known as Phospholipase A2 (PLA2), PLA2A, PPLA2, which belongs to the phospholipase A2 family. PLA2G1B is activated by trypsin cleavage in the duodenum and can also be activated by thrombin or autocatalytically. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides, this releases glycerophospholipids and arachidonic acid that serve as the precursors of signal molecules.
• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Ala23
• Form: Lyophilized from 0.22 μm filtered solution in 20 mM Hepes, 150 mM NaCl, pH7.0, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 15.0 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE).
• Protein length: Ala23-Ser148
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PLA2G1B
• Official Symbol: PLA2G1B
• Synonyms: PLA2G1B; phospholipase A2, group IB (pancreas); PLA2, PLA2A, PPLA2; phospholipase A2; group IB phospholipase A2; phosphatidylcholine 2-acylhydrolase 1B; PLA2; PLA2A; PPLA2; MGC119834; MGC119835
• Gene ID: 5319
• mRNA Refseq: NM_000928
• Protein Refseq: NP_000919
• MIM: 172410
• UniProt ID: P04054