Active Recombinant Human CD3E&CD3D, His&flag-tagged

• Cat.No.: CD3E&CD3D-219H
• Product Overview: Recombinant Human CD3E & CD3D heterodimer protein (Human CD3E & CD3D heterodimer) Met1-Asp126 (CD3E,NP_000724.1) & Phe 22 - Ala 105 (CD3D,AAH39035) was produced in human 293 cells (HEK293).

Specification

• Source: HEK293
• Species: Human
• Tag: His&flag
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human CD3D & CD3E Heterodimer Protein at 2 μg/ml (100 μl/well) can bind OKT3-mIgG2a. The EC50 of OKT3-mIgG2a is 7-28 ng/ml.
• Molecular Mass: The recombinant heterodimer of human CD3D&CD3E comprises 271 (126+145) amino acids and has a calculated molecular mass of 31.05 (14.4+16.65)KDa. As a result of glycosylation, the apparent molecular mass of human CD3D&CD3E heterodimer is approximately 20, 22 and 26 KDa in SDS-PAGE under reducing conditions.
• Protein Length: Met1-Ala105&Met1-Asp126
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publication:
  Efficient tumor killing and minimal cytokine release with novel T-cell agonist bispecific antibodies (2019)