Active Recombinant Human FCER1A, His-tagged, Biotinylated

• Cat.No.: FCER1A-193H
• Product Overview: Human FCER1A Protein, Biotinylated (Biotinylated rh FCER1A) Val 26 - Gln 205 (Accession # NP_001992), was labeled with biotin based on rh FCER1A Protein, which was produced in human 293 cells (HEK293).

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Immunoglobulin E, Human Plasma at 5 μg/mL (100 μL/well) can bind Human Fc epsilon RI alpha, His Tag with a linear range of 0.4-3 ng/mL.
• Molecular Mass: The protein has a calculated MW of 22.9 kDa. The protein migrates as 37-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Val 26 - Gln 205
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
  >90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: FCER1A Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide [ Homo sapiens ]
• Official Symbol: FCER1A
• Synonyms: FCER1A; Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; FCE1A; high affinity immunoglobulin epsilon receptor subunit alpha; Fc-epsilon RI-alpha; Fc epsilon RI alpha-chain; igE Fc receptor subunit alpha; Fc IgE receptor, alpha polypeptide; high affinity immunoglobulin epsilon receptor alpha-subunit; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide; FcERI
• Gene ID: 2205
• mRNA Refseq: NM_002001
• Protein Refseq: NP_001992
• MIM: 147140
• UniProt ID: P12319