| Description : |
Phospholipase D is a phospholipid hydrolyzing enzyme and an important component of receptor-mediated signal transduction responses and regulated secretion. Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. Phospholipase D is involved in conferring drought susceptibility in peanuts, which increases the risk of aflatoxin contamination. |
| Source : |
Arachis hypogaea (peanut) |
| Species : |
Arachis hypogaea (peanut) |
| Form : |
lyophilized powder. Partially purified, lyophilized powder containing buffer salts. |
| Bio-activity : |
≥60 units/mg protein |
| Purity : |
Protein, ~30% |
| Unit Definition : |
One unit will liberate 1.0 μmol of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C. |
| Applications : |
Research has shown ADP-ribosylation factor regulation of phospholipase D is important in the release of nascent secretory vesicles from the trans-Golgi network. It has also been used in a study to investigate stimulation of Na+-Ca2+ exchange activity in canine cardiac sarcolemmal vesicals. |
| Notes : |
Protein determined using biuret, unless otherwise indicated. |
| Storage : |
Store at -20 °C |
