Recombinant Human TP53, His-tagged

• Cat.No.: TP53-58H
• Product Overview: Recombinant Human p53, transcript variant 1, was expressed in E. coli from human cDNA (accession number NM_000546.5) with an N-terminal polyhistidine tag.

Specification

• Description: p53 is a tumor suppressor protein functioning as a sequence-specific transcription factor important for cell cycle arrest, apoptosis, and DNA-damage response.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 50 mM Tris-HCl pH 8, 200 mM NaCl, 5 mM beta-mercaptoethanol and 5% glycerol.
• Molecular Mass: 53 kDa
• Applications: In vitro transcription, protein-protein interactions and protein-DNA interaction studies. A recommended starting point for in vitro transcription assays is 20-100 ng per reaction.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Publication:
  Development of cell-based high throughput luminescence assay for drug discovery in inhibiting OCT4/DNA-PKcs and OCT4–MK2 interactions (2021)

Gene Information

• Gene Name: TP53 tumor protein p53 [ Homo sapiens ]
• Official Symbol: TP53
• Synonyms: TP53; tumor protein p53; cellular tumor antigen p53; LFS1; Li Fraumeni syndrome; p53; antigen NY-CO-13; mutant p53 protein; phosphoprotein p53; p53 tumor suppressor; truncated p53 protein; tumor suppressor TP53; transformation-related protein 53; P53; TRP53; FLJ92943
• Gene ID: 7157
• mRNA Refseq: NM_000546
• Protein Refseq: NP_000537
• MIM: 191170
• UniProt ID: P04637
• Chromosome Location: 17p13.1
• Pathway: Activation of BH3-only proteins, organism-specific biosystem; Activation of NOXA and translocation to mitochondria, organism-specific biosystem; Activation of PUMA and translocation to mitochondria, organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem
• Function: ATP binding; DNA binding; DNA strand annealing activity; MDM2 binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; RNA polymerase II transcription factor binding; chaperone binding; chromatin binding; copper ion binding; damaged DNA binding; enzyme binding; histone acetyltransferase binding; histone deacetylase regulator activity; identical protein binding; metal ion binding; p53 binding; protease binding; protein N-terminus binding; protein binding; protein heterodimerization activity; protein kinase binding; protein phosphatase 2A binding; sequence-specific DNA binding transcription factor activity; transcription factor binding; transcription regulatory region DNA binding; ubiquitin protein ligase binding; zinc ion binding