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Recombinant Human TAF1, His&FLAG-tagged

Cat.No. : TAF1-78H
Product Overview : Recombinant Human TAF1 corresponding to amino acids 1398-1524 that contains the bromodomain sequences of TAF1 (accession number NP_004597.2) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 21 kDa.
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Description : TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex. TAF1 contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. TAF1 also contains bromodomains for interaction with other proteins. The bromodomain functions as a "reader" of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TAF1 has been shown to phosphorylate threonine 55 of p53 which leads to MDM2-mediated degradation of p53. TAF1 also phosphorylates GTF2A1 and GTF2F1 on serine residues. In addition, TAF1 possesses DNA-binding activity and is essential for progression of the G1 phase of the cell cycle.
Source : E. coli
Species : Human
Tag : His&FLAG
Form : 25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol.
Applications : Binding assays, inhibitor screening, and selectivity profiling.
Storage : Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Concentration : 5.5 mg/ml
Gene Name : TAF1 TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa [ Homo sapiens ]
Official Symbol : TAF1
Synonyms : TAF1; TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa; BA2R, CCG1, CCGS, dystonia 3 (with Parkinsonism) , DYT3, TAF2A, TATA box binding protein (TBP) associated factor, RNA polymerase II, A, 250kD; transcription initiation factor TFIID subunit 1; DYT3/TAF1; KAT4; NSCL2; TAFII250; TAFII-250; TAF(II)250; cell cycle gene 1 protein; cell cycle, G1 phase defect; TBP-associated factor 250 kDa; transcription factor TFIID p250 polypeptide; complementation of cell cycle block, G1-to-S; transcription initiation factor TFIID 250 kDa subunit; OF; XDP; BA2R; CCG1; CCGS; DYT3; P250; TAF2A; N-TAF1;
Gene ID : 6872
mRNA Refseq : NM_004606
Protein Refseq : NP_004597
MIM : 313650
UniProt ID : P21675
Chromosome Location : Xq13.1
Pathway : Basal transcription factors, organism-specific biosystem; Basal transcription factors, conserved biosystem; Disease, organism-specific biosystem; Gene Expression, organism-specific biosystem; HIV Infection, organism-specific biosystem; HIV Life Cycle, organism-specific biosystem; HIV-1 Transcription Initiation, organism-specific biosystem;
Function : ATP binding; TBP-class protein binding; histone acetyl-lysine binding; histone acetyltransferase activity; kinase activity; nucleotide binding; p53 binding; protein binding; protein serine/threonine kinase activity; sequence-specific DNA binding; transcription coactivator activity; transcription factor binding; contributes_to transcription regulatory region DNA binding; transferase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What are the mechanisms by which TAF1 modulates the expression of specific sets of genes? 11/17/2022

TAF1 specifically modulates the expression of certain genes, impacting diverse cellular functions and processes.

How does TAF1 (TATA-Box Binding Protein Associated Factor 1) regulate transcription initiation by RNA polymerase II? 05/03/2022

TAF1 is pivotal in initiating transcription by RNA polymerase II, regulating the early steps of gene expression.

How does TAF1 contribute to the regulation of gene expression in response to cellular signals and stress? 09/30/2021

TAF1 responds to cellular signals and stress, adapting gene expression to meet cellular needs and challenges.

How does TAF1 influence chromatin remodeling and accessibility of DNA for transcription? 04/21/2021

TAF1 plays a role in chromatin remodeling, making DNA accessible for transcription and regulating gene activity.

How do alterations in TAF1 expression or function affect the cell cycle and apoptosis? 03/11/2019

Changes in TAF1 can influence key cellular processes like the cell cycle and programmed cell death.

What role does TAF1 play in the assembly and function of the transcription factor IID (TFIID) complex? 08/05/2018

TAF1 is integral to the TFIID complex, essential for accurate and efficient transcription initiation.

What is the impact of TAF1 mutations on neurodevelopmental disorders and intellectual disabilities? 02/06/2018

Mutations in TAF1 are linked to neurodevelopmental disorders, affecting brain development and function.

Customer Reviews (3)

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Reviews
10/20/2021

    Expertise in peptide synthesis, accelerates our peptide-based research.

    04/15/2019

      Swift protein quantification, improves data reliability in our work.

      11/26/2018

        Reliable protein crystallography, key for our structural biology research.

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