Human Hepatocyte Growth Factor(precursor, rDNA derived) Reference standard

• Cat.No.: HGF-157H
• Product Overview: This consists of a batch of ampoules containing single- chain hepatocyte growth factor/scatter factor (HGF/SF) precursor expressed in Sf21 insect cells using a baculovirus expression system. The material is intended to serve as a standard for the bioassay or immunoassay of single-chain HGF/SF precursor preparations only. For assays of heterodimeric HGF/SF, the corresponding standard Human Hepatocyte Growth Factor(rDNA derived) should be used.

Specification

• Source: Sf21 insect cells
• Species: Human
• Enzyme Unit: 2000 International Units (IU) per ampoule.
• Usage: No attempt should be made to weigh out any portion of the freeze-dried material prior to reconstitution For practical purposes each ampoule contains the same quantity of HGF/SF. The entire content of each ampoule should be completely dissolved in an accurately measured amount of buffer solution. The use of water to reconstitute ampoule contents is not recommended. It is recommended that, when possible, buffer containing carrier protein should be used to minimize loss by surface adsorption. The buffer should be compatible with the assay system used.
• Notes: This preparation is not for administration to humans. The material is not of human or bovine origin. As with all materials of biological origin, this preparation should be regarded as potentially hazardous to health. It should be used and discarded according to your own laboratorys safety procedures. Such safety procedures should include the wearing of protective gloves and avoiding the generation of aerosols. Care should be exercised in opening ampoules or vials, to avoid cuts.
• Stability: Reference materials are held at Creative Biomart within assured, temperature- controlled storage facilities and they should be stored on receipt as indicated on the label. Accelerated degradation studies have indicated that this material is suitably stable, when stored at -20°C or below, for the assigned values to remain valid until the material is withdrawn or replaced. These studies have also shown that the material is suitably stable for shipment at ambient temperature without any effect on the assigned values. Users who have data supporting any deterioration in the characteristics of any reference preparation are encouraged to contact Creative Biomart.
• Storage: The ampoules are shipped at ambient temperature. Unopened ampoules should be stored at -20 °C in the dark. Freezing and reuse of reconstituted material should be avoided unless this can be validated for the particular laboratory, storage and assay conditions. Repeated freezing and thawing should be avoided. The ampoules do not contain bacteriostat and solutions of the ampouled material should not be assumed to be sterile. Please note: because of the inherent stability of lyophilized material, Creative Biomart may ship these materials at ambient temperature.

Gene Information

• Gene Name: HGF hepatocyte growth factor (hepapoietin A; scatter factor) [ Homo sapiens ];
• Official Symbol: HGF
• Synonyms: HGF; hepatocyte growth factor (hepapoietin A; scatter factor); deafness, autosomal recessive 39 , DFNB39; hepatocyte growth factor; F TCF; fibroblast derived tumor cytotoxic factor; hepatopoietin A; HGFB; HPTA; lung fibroblast derived mitogen; scatter factor; SF; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor; F-TCF; DFNB39
• Gene ID: 3082
• mRNA Refseq: NM_000601
• Protein Refseq: NP_000592
• MIM: 142409
• UniProt ID: P14210
• Chromosome Location: 7q21.1
• Pathway: Arf6 signaling events, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Direct p53 effectors, organism-specific biosystem; FGF signaling pathway, organism-specific biosystem; Focal Adhesion, organism-specific biosystem
• Function: catalytic activity; growth factor activity; protein binding; protein heterodimerization activity; NOT serine-type endopeptidase activity