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Cat. No.
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TrxR-77Y
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Product Overview
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Recombinant Yeast Thioredoxin Reductase (NADPH) produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 36 kDa. Thioredoxin Reductase is purified by proprietary chromatographic techniques.
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Introduction
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Thioredoxin reductase (TrxR/NTR), an enzyme belonging to the flavoprotein family of pyridine nucleotide-disulfide oxidoreductases. Thioredoxin reductase (TrxR), a component of the thioredoxin system, including thioredoxin (Trx) and NADPH, catalyzes the transfer of electrons from NADPH to Trx, acts as a reductant of disulfide-containing proteins and participates in the defense system against oxidative stresses.
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Synonyms
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Thioredoxin Reductase (NADPH); NTR; TrxR; EC 1.8.1.9; GRIM-12; KDRF; MGC9145; TR; TR1; TRXR1; TXNR; Trxr1; oxidoreductase; Gene associated with retinoid-IFN-induced mortality 12 protein; KM-102-derived reductase-like factor; Thioredoxin reductase TR1; thioredoxin reductase 1; thioredoxin reductase GRIM-12; thioredoxin-disulfide reductase; NADP-thioredoxin reductase; NADPH-thioredoxin reductase; thioredoxin reductase (NADPH); NADPH2:oxidized thioredoxin oxidoreductase; thioredoxin:NADP+ oxidoreductase.
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Source
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Escherichia Coli.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Purity
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Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
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Formulation
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Each mg of protein contains 20 mM phosphate buffer pH 7.4 and 0.15 M sodium chloride.
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Solubility
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It is recommended to reconstitute the lyophilized NTR in sterile 18 MΩ-cm H2O.
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Biological Activity
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The specific activity was found to be 5 IU/mg.
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Unit Definition
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One unit equals the change in absorbance at 412 nm per minute at 25°C using 0.2 mM NADPH containing 5 mM DTNB (pH 7.0)
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Stability
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NTR although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Please prevent freeze thaw cycles.
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Download Datasheet:
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