Recombinant Human Glucocorticoid receptor LBD/Hsp90 complex, GST-tagged

• Cat.No.: NR3C1-104H
• Product Overview: Recombinant Human GST-tagged Glucocorticoid receptor ligand-binding domain (GR-LBD) consists of a 218 a.a. GST tag, a 30 a.a. flexible linker and a 275 a.a. GR-LBD, Acc# NP_000167, amino acids 503-777. It was produced in complex with human Hsp90 to facilitate native protein conformation and advanced performance in ligand binding and coactivator peptide recruitment assays. GST tagged GR-LBD, human Hsp90 and human Hsc70 are major components of the complex.

Specification

• Source: Sf9 insect cells
• Species: Human
• Tag: GST
• Form: 20 mM Na-P pH 8.0, 40 mM KCl,20 mM Na2MoO4, 5 mM DTT, 7 mM reduced glutathione, 15% glycerol.
• Purity: >80%
• Applications: Ligand binding and coactivator peptide recruitment assays.
• Storage: Immediately store at -80℃. Avoid freeze-thaw cycles.While working, please keep sample on ice.

Gene Information

• Gene Name: NR3C1 nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) [ Homo sapiens ]
• Official Symbol: NR3C1
• Synonyms: NR3C1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); GRL, nuclear receptor subfamily 3, group C, member 1; glucocorticoid receptor; GR; glucocorticoid nuclear receptor variant 1; GCR; GRL; GCCR
• Gene ID: 2908
• mRNA Refseq: NM_000176
• Protein Refseq: NP_000167
• UniProt ID: P04150
• Chromosome Location: 5q31-q32
• Pathway: Adipogenesis, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; Circadian Clock, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem
• Function: Hsp70 protein binding; Hsp90 protein binding; chromatin binding; double-stranded DNA binding; glucocorticoid receptor activity; metal ion binding; protein binding; protein heterodimerization activity; protein homodimerization activity; receptor activity; receptor tyrosine kinase binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; steroid binding; zinc ion binding