Active Native Calf Alkaline Phosphatase
Cat.No. : | ALPL-66C |
- Specification
- Gene Information
- Related Products
Description : | Alkaline Phosphatase (ALP) is a hydrolase enzyme responsible for removing phosphate groups in the 5- and 3- positions from many types of molecules, including nucleotides, proteins, and alkaloids. In humans, alkaline phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta. The optimal pH for the enzyme activity is pH 10 in standard conditions. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3 untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. |
Source : | Calf Intestine |
Species : | Calf |
Form : | Freeze Dried |
Bio-activity : | 3,000-6,000 U/mg protein |
Purity : | Purity by SDS-PAGE: ≥98% |
Usage : | For Research Use Only! Not For Use in Humans. |
Notes : | Centrifuge the vial prior to opening |
Storage : | -20°C |
Storage Buffer : | Lyophilized without any additives. |
Reconstitution : | Reconstitute in distilled water or dilute buffer. |
Shipping : | Gel pack |
Gene Name : | ALPL alkaline phosphatase, liver/bone/kidney [ Bos taurus ] |
Official Symbol : | ALPL |
Synonyms : | TNS-AP; alkaline phosphatase, tissue-nonspecific isozyme; TNSALPAP-TNAPliver/bone/kidney isozyme; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase tissue non-specific isoform |
Gene ID : | 280994 |
mRNA Refseq : | NM_176858 |
Protein Refseq : | NP_789828 |
UniProt ID : | P09487 |
Chromosome Location : | chromosome: 2 |
Pathway : | Endochondral Ossification, organism-specific biosystem; Folate biosynthesis, organism-specific biosystem; Metabolic pathways, organism-specific biosystem |
Function : | alkaline phosphatase activity; metal ion binding; pyrophosphatase activity |
Products Types
◆ Recombinant Protein | ||
ALPL-123C | Recombinant Cattle ALPL Protein, His-tagged | +Inquiry |
ALPL-138R | Recombinant Rhesus Macaque ALPL Protein, His (Fc)-Avi-tagged | +Inquiry |
ALPL-001H | Recombinant Human ALPL Protein, His-tagged | +Inquiry |
ALPL-1994H | Recombinant Human ALPL Protein, MYC/DDK-tagged | +Inquiry |
ALPL-2458H | Recombinant Human ALPL Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Native Protein | ||
ALPL-25H | Active Native Human ALPL Protein | +Inquiry |
ALPL-8004H | Native Human Liver Alkaline Phosphatase | +Inquiry |
ALPL-5324H | Active Native Human Alkaline Phosphatase | +Inquiry |
◆ Lysates | ||
ALPL-3092HCL | Recombinant Human ALPL cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
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Q&As (10)
Ask a questionALPL exhibits tissue-specific expression, with high levels in mineralizing tissues such as bones and teeth, but lower levels in other organs.
ALPL activity and expression undergo dynamic changes during development and aging, reflecting the physiological demands for mineralization.
ALPL activity can be modulated by factors such as pyrophosphate, calcium, and inhibitors like levamisole, which impact mineralization outcomes.
ALPL plays a critical role in mineralization processes by generating inorganic phosphate, an essential component of hydroxyapatite crystals in bones and teeth.
ALPL can serve as a diagnostic marker for hypophosphatasia and related mineralization disorders, and therapeutic strategies targeting ALPL are being explored.
ALPL deficiency or dysregulation is associated with hypophosphatasia, a genetic disorder characterized by impaired mineralization and skeletal abnormalities.
Genetic variations or mutations in the ALPL gene can lead to altered enzyme activity, affecting mineralization and contributing to disease phenotypes.
Manipulating ALPL activity and function holds promise for promoting bone regeneration and dental tissue engineering, enhancing mineralization processes and tissue formation.
ALPL regulates molecular interactions and signaling pathways, including Wnt/尾-catenin, BMP, and FGF signaling, to orchestrate mineralization processes.
ALPL gene expression is regulated by transcription factors, epigenetic modifications, and signaling pathways involved in skeletal development and mineralization.
Customer Reviews (3)
Write a reviewEfficient target protein purification.
Reliable antibody for Western blotting.
Robust enzyme activity measurement.
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