Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Active Native Calf Alkaline Phosphatase

Cat.No. : ALPL-66C
  • Specification
  • Gene Information
  • Related Products
Description : Alkaline Phosphatase (ALP) is a hydrolase enzyme responsible for removing phosphate groups in the 5- and 3- positions from many types of molecules, including nucleotides, proteins, and alkaloids. In humans, alkaline phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta. The optimal pH for the enzyme activity is pH 10 in standard conditions. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3 untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.
Source : Calf Intestine
Species : Calf
Form : Freeze Dried
Bio-activity : 3,000-6,000 U/mg protein
Purity : Purity by SDS-PAGE: ≥98%
Usage : For Research Use Only! Not For Use in Humans.
Notes : Centrifuge the vial prior to opening
Storage : -20°C
Storage Buffer : Lyophilized without any additives.
Reconstitution : Reconstitute in distilled water or dilute buffer.
Shipping : Gel pack
Gene Name : ALPL alkaline phosphatase, liver/bone/kidney [ Bos taurus ]
Official Symbol : ALPL
Synonyms : TNS-AP; alkaline phosphatase, tissue-nonspecific isozyme; TNSALPAP-TNAPliver/bone/kidney isozyme; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase tissue non-specific isoform
Gene ID : 280994
mRNA Refseq : NM_176858
Protein Refseq : NP_789828
UniProt ID : P09487
Chromosome Location : chromosome: 2
Pathway : Endochondral Ossification, organism-specific biosystem; Folate biosynthesis, organism-specific biosystem; Metabolic pathways, organism-specific biosystem
Function : alkaline phosphatase activity; metal ion binding; pyrophosphatase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (10)

Ask a question
What is the tissue-specific expression pattern of ALPL in different organs and cell types? 12/11/2022

ALPL exhibits tissue-specific expression, with high levels in mineralizing tissues such as bones and teeth, but lower levels in other organs.

How does ALPL activity and expression change during development and aging? 09/09/2022

ALPL activity and expression undergo dynamic changes during development and aging, reflecting the physiological demands for mineralization.

Can ALPL activity be modulated by specific factors or inhibitors, and how does this affect mineralization? 06/23/2021

ALPL activity can be modulated by factors such as pyrophosphate, calcium, and inhibitors like levamisole, which impact mineralization outcomes.

How does ALPL contribute to mineralization processes, such as bone formation and tooth development? 10/26/2019

ALPL plays a critical role in mineralization processes by generating inorganic phosphate, an essential component of hydroxyapatite crystals in bones and teeth.

Can ALPL serve as a diagnostic marker or therapeutic target for mineralization-related diseases? 02/27/2019

ALPL can serve as a diagnostic marker for hypophosphatasia and related mineralization disorders, and therapeutic strategies targeting ALPL are being explored.

How does ALPL deficiency or dysregulation contribute to the pathogenesis of skeletal and dental disorders, such as hypophosphatasia? 08/21/2018

ALPL deficiency or dysregulation is associated with hypophosphatasia, a genetic disorder characterized by impaired mineralization and skeletal abnormalities.

What are the effects of genetic variations or mutations in the ALPL gene on enzyme activity and mineralization phenotypes? 12/19/2017

Genetic variations or mutations in the ALPL gene can lead to altered enzyme activity, affecting mineralization and contributing to disease phenotypes.

How can ALPL activity and function be manipulated to enhance bone regeneration and dental tissue engineering? 08/22/2017

Manipulating ALPL activity and function holds promise for promoting bone regeneration and dental tissue engineering, enhancing mineralization processes and tissue formation.

What are the downstream signaling pathways and molecular interactions regulated by ALPL in mineralization processes? 05/18/2017

ALPL regulates molecular interactions and signaling pathways, including Wnt/尾-catenin, BMP, and FGF signaling, to orchestrate mineralization processes.

What are the regulatory mechanisms controlling ALPL gene expression and enzymatic activity? 01/09/2017

ALPL gene expression is regulated by transcription factors, epigenetic modifications, and signaling pathways involved in skeletal development and mineralization.

Customer Reviews (3)

Write a review
Reviews
04/22/2022

    Efficient target protein purification.

    04/05/2018

      Reliable antibody for Western blotting.

      12/20/2017

        Robust enzyme activity measurement.

        Ask a Question for All ALPL Products

        Required fields are marked with *

        My Review for All ALPL Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends