Native Human Clusterin

• Cat.No.: CLU-67H

Specification

• Description: Apoliprotein J (APO-J), also named Clusterin, is a 75-80 kD disulfide-linked heterodimeric protein. The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the a and b chains. These are assembled in anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil a-helices and three predicted amphipathic a-helices. Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.
• Source: Plasma
• Species: Human
• Form: Lyophilized protein
• Molecular Mass: 75-80 kDa
• Endotoxin: <0.1>
• Purity: Purity by SDS-PAGE: ≥95%; Purity by HPLC: ≥95%
• Usage: For Research Use Only! Not For Use in Humans.
• Notes: Centrifuge the vial prior to opening
• Storage: -20°C
• Storage Buffer: Sterile filtered and lyophilized from PBS, pH 7.5.
• Reconstitution: Reconstitute in dH?O to a working volume of 0.5 mg/ml and let the lyophilized pellet dissolve completely.
• Shipping: Blue ice

Gene Information

• Gene Name: CLU clusterin [ Homo sapiens ]
• Official Symbol: CLU
• Synonyms: CLU; clusterin; APOJ, CLI, clusterin (complement lysis inhibitor, SP 40,40, sulfated glycoprotein 2, testosterone repressed prostate message 2, apolipoprotein J); apolipoprotein J; complement lysis inhibitor; KUB1; SGP 2; SP 40; sulfated glycoprotein 2
• Gene ID: 1191
• mRNA Refseq: NM_001831
• Protein Refseq: NP_001822
• MIM: 185430
• UniProt ID: P10909
• Chromosome Location: 8p21-p12
• Pathway: Hemostasis, organism-specific biosystem; Platelet activation, signaling and aggregation, organism-specific biosystem; Platelet degranulation, organism-specific biosystem; Response to elevated platelet cytosolic Ca2+, organism-specific biosystem; Validated targets of C-MYC transcriptional repression, organism-specific biosystem
• Function: NOT ATPase activity; misfolded protein binding; misfolded protein binding; protein binding; ubiquitin protein ligase binding