Active Native Bovine Thrombin

• Cat.No.: TIMP1-91B

Specification

• Description: Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.
• Source: Bovine plasma
• Species: Bovine
• Form: Lyophilized
• Bio-activity: 90-250 U/mg of lyophilized powder.
• Molecular Mass: 37 kDa
• Purity: Purity by SDS-PAGE: ≥98%
• Usage: For Research Use Only! Not For Use in Humans.
• Notes: Centrifuge the vial prior to opening
• Storage: -20°C
• Storage Buffer: Sterile filtered and lyophilized with Mannitol and Sodium Chloride.
• Reconstitution: Reconstitute in sterile water (100 U/ml) with 0.9% NaCl. It forms a clear solution.
• Shipping: Gel pack

Gene Information

• Gene Name: TIMP1 TIMP metallopeptidase inhibitor 1 [ Bos taurus ]
• Official Symbol: TIMP1
• Synonyms: metalloproteinase inhibitor 1; EG-1TIMP-1embryogenin-1; tissue inhibitor of metalloproteinases 1; tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)
• Gene ID: 282092
• mRNA Refseq: NM_174471
• Protein Refseq: NP_776896
• UniProt ID: P20414
• Chromosome Location: chromosome: X
• Pathway: Activation of Matrix Metalloproteinases, organism-specific biosystem; Hemostasis, organism-specific biosystem; Platelet degranulation, organism-specific biosystem
• Function: cytokine activity; metal ion binding; metalloendopeptidase inhibitor activity