Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Active Native Human C7 Protein

Cat.No. : C7-102H
  • Specification
  • Gene Information
  • Related Products
Description : Native human C7 is a naturally glycosylated protein composed of a single polypeptide chain of 92,400 Da. C7 is essential for formation of the membrane attack complex (MAC) and is activated non-proteolytically by binding to recently-formed C5b,C6 complexes at the cell membrane. Each pathway of complement activation generates proteolytic enzyme complexes (C3/C5 convertases) which are bound to the target surface (Ross, G.D. (1986); Law, S.K.A. and Reid, K.B.M. (1995)). These enzymes cleave a peptide bond in the larger alpha chain of C5 releasing the anaphylatoxin C5a and activating C5b. This is the only proteolytic step in the assembly of the C5b-9 complex. C5b is unstable, but it remains bound to the activating complex for a brief time (~2 min) during which it either binds a single C6 from the surrounding fluid or decays and is no longer capable of forming MAC. The C5b,6 complex may also remain bound to the C3/C5 convertase where the binding of a single C7 exposes a membrane-binding region and C5b,6,7 can partially insert into the bilipid layer of the target cell. Up to this point the complex may diffuse away from the target cell and enter the membrane of a nearby cell. This is called bystander lysis or "reactive lysis" and can be a significant source of pathology. Each C5b-7 complex can bind one C8 protein molecule which results in the complex inserting more firmly into the membrane. This complex binds C9 and each bound C9 can bind another C9 initiating formation of a ring structure containing up to 18 C9 molecules (Podack, E.R. (1984)). C5b-9 complexes with one or more C9 are referred to as the Membrane Attack Complex (MAC) of complement. Not all C5b-8 complexes have complete rings of C9 with the average being only three C9 per C5b-8 complex. Completed protein rings of C9 form the pores seen on electron micrographs and they result in leakage of metabolites and small proteins out of the cell as well as movement of water into the cell. If sufficient numbers are inserted into a cell membrane then water flowing into the cell, due to osmotic pressure, will rupture the cell membrane allowing the entire contents of the target cell (or a bystander cell) to be released. Either process may result in cell death. Originally it was thought that this required only one C5b-9 complex per cell (referred to as the "one hit theory" of lysis (Rommel F.A. and Mayer, M.M. (1973)), but this is probably not correct. For example, an erythrocyte requires ~850 C5b-9 complexes, as measured by the number of C7 molecules, for lysis to occur (Bauer, J. et al. (1979)). Host cells protected from MAC by CD59 require sufficient numbers of C5b-9 to tie up all the CD59 and then approximately 850 C5b-9 in addition. Lysis of nucleated cells requires many more C5b-9 complexes due to their size and due to the presence of multiple defense mechanisms in such cells.
Source : Normal human serum (shown by certified tests to be negative for HBsAg, HTLV-I/II, STS and for antibodies to HCV, HIV-1 and HIV-II).
Species : Human
Form : Liquid
Bio-activity : >95% versus normal human serum standard
Molecular Mass : 92,400 Da (single chain)
Purity : >90% by SDS-PAGE
Characteristic : The molecular weight of C7 is 92,400 Da and it is composed of a single polypeptide chain. The pI of C7 is heterogeneous from 6.0 to 6.5.
Usage : For research use only. Not for human or drug use.
Storage : -70°C or below. Avoid freeze/thaw.
Concentration : 1.0 mg/mL (see Certificate of Analysis for exact conc.)
Storage Buffer : 10 mM Sodium phosphate, 145 mM NaCl, pH 7.3
Preservative : None, 0.22 μm filtered
Warning : Use normal precautions for handling human blood products.
Gene Name : C7 complement component 7 [ Homo sapiens ]
Official Symbol : C7
Synonyms : C7; complement component 7; complement component C7;
Gene ID : 730
mRNA Refseq : NM_000587
Protein Refseq : NP_000578
MIM : 217070
UniProt ID : P10643
Chromosome Location : 5
Pathway : Complement Activation, Classical Pathway, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Complement cascade, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem;

Products Types

◆ Recombinant Protein
C7-7369H Recombinant Human C7 protein(Met1-Gln843), His-tagged +Inquiry
C7-0129H Recombinant Human C7 Protein, GST-Tagged +Inquiry
C7-823P Recombinant Pig C7 protein, His & GST-tagged +Inquiry
C7-0585H Recombinant Human C7 Protein (Ser122-His456), His-tagged +Inquiry
C7-824R Recombinant Rat C7 protein, His & T7-tagged +Inquiry

See All C7 Recombinant Protein

◆ Native Protein
C7-56H Native Human Complement C7 +Inquiry

See All C7 Native Protein

◆ Lysates
C7-1425HCL Recombinant Human C7 cell lysate +Inquiry
C7-1500HCL Recombinant Human C7 cell lysate +Inquiry

See All C7 Lysates

Related Gene

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (0)

Ask a question

Customer Reviews (0)

Write a review

Ask a Question for All C7 Products

Required fields are marked with *

My Review for All C7 Products

Required fields are marked with *

0

Inquiry Basket

cartIcon
logo

FOLLOW US

Terms and Conditions        Privacy Policy

Copyright © 2024 Creative BioMart. All Rights Reserved.

Contact Us

  • /

Stay Updated on the Latest Bioscience Trends