Native Human Complement C3c

• Cat.No.: C3-194H

Specification

• Description: Complement C3 (C3) is the most abundant component of the complement system, with levels in human plasma of ~1.2-1.5 mg/ml. It is central to the activation of both the classical and alternative complement pathways, both of which lead to the proteolytic processing of C3 to C3a and C3b. C3 exists as a heterodimer with molecular masses of 115 kDa and 75 kDa, although it is readily processed to smaller fragments (C3a, C3b, C3c) as evidenced by the molecular weight description below. The current protocol yields C3c, however intact C3 and/or partially processed C3a/C3b may potentially be purified if certain precautions are taken during processing (ie, processing at 4°C, protease inhibitors throughout processing).
• Source: Human Plasma
• Species: Human
• Form: Frozen
• Bio-activity: Not biologically active
• Molecular Mass: 138000
• Purity: >=95% by SDS-PAGE Reacts to C3 antisera on Western Blot Negative against C4 antisera on Western Blot
• Usage: For research use or for use in further manufacturing.
• Quality Control Test: Shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
• Stability: >1 year
• Storage: <= -70°c - avoid>
• Storage Buffer: Phosphate Buffered Saline, pH 7.4

Gene Information

• Gene Name: C3 complement component 3 [ Homo sapiens ]
• Official Symbol: C3
• Synonyms: C3; complement component 3; complement C3; CPAMD1; complement component C3; acylation-stimulating protein cleavage product; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; ASP; AHUS5; ARMD9
• Gene ID: 718
• mRNA Refseq: NM_000064
• Protein Refseq: NP_000055
• MIM: 120700
• UniProt ID: P01024
• Chromosome Location: 19p13.3-p13.2
• Pathway: Activation of C3 and C5, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Alternative complement activation, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Complement Activation, Classical Pathway, organism-specific biosystem
• Function: endopeptidase inhibitor activity; protein binding; receptor binding