Recombinant Chicken Lactate Dehydrogenase B

• Cat.No.: LDHB-82C
• Product Overview: Recombinant chicken LDH-B produced inE.Coliis cloned from cDNA library of chicken heart.

Specification

• Cat. No.: LDHB-82C
• Description: Lactate dehydrogenase (LDH) is an enzyme (EC 1.1.1.27) present in a wide variety of organisms, including plants and animals. A tetrameric enzyme that catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
• Source: Escherichia Coli.
• Physical Appearance: Sterile lyophilized powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC (b) Analysis by SDS-PAGE.
• Formulation: The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate.
• Biological Activity: The specific activity was found to be 200 IU/mg.
• Unit Definition: One unit is defined as 1 μmol of NAD+ production per minute under the assay conditions (25°C, pH 7.0). Both transaminase activities include α-hydroxyglutarate dehydrogenase activity.
• Stability: Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: LDHB lactate dehydrogenase B [ Gallus gallus ]
• Synonyms: LDHB; lactate dehydrogenase B; L-lactate dehydrogenase B; EC 1.1.1.27
• Gene ID: 373997
• mRNA Refseq: NM_204177
• Protein Refseq: NP_989508
• UniProt ID: P00337
• Chromosome Location: 1
• Pathway: Cysteine and methionine metabolism; Glycolysis/Gluconeogenesis; Metabolic pathways; Propanoate metabolism; Pyruvate metabolism
• Function: L-lactate dehydrogenase activity; binding; oxidoreductase activity