Active Recombinant Human CUL2/RBX1 protein

• Cat.No.: CUL2-133H
• Product Overview: Recombinant Human CUL2/RBX1 was coexpressed in Insect cells.

Specification

• Source: Insect cells
• Species: Human
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: E3 Ligase Assay: The activity of Cul2/Rbx1 was validated indirectly through its ability to act as a substrate for Neddylation in the presence of the NEDD8 E3 ligase His-DCNL2 and thioester-loaded His-UBE2M-NEDD8. Incubation of Cul2/Rbx1 and thioester loaded His-UBE2M-NEDD8 in the presence or absence of His-DCNL2 at 4˚C was compared at two time points T0 and T2 minutes. Neddylation of the Cul2 subunit in the presence of His-DCNL2 was demonstrated.
• Molecular Mass: Cul2=~87.1kDa; Rbx1=~12.3kDa
• Purity: >75% by InstantBlue™ SDS-PAGE
• Stability: 12 months at -70 centigrade; aliquot as required
• Storage: Store product at –70 centigrade
• Concentration: 0.5mg/ml

Gene Information

• Gene Name: CUL2 cullin 2 [ Homo sapiens ]
• Official Symbol: CUL2
• Synonyms: CUL2; cullin 2; cullin-2; CUL-2; MGC131970
• Gene ID: 8453
• mRNA Refseq: NM_001198777
• Protein Refseq: NP_001185706
• MIM: 603135
• UniProt ID: Q13617
• Chromosome Location: 10p11.2
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; ECV complex, organism-specific biosystem; Hypoxic and oxygen homeostasis regulation of HIF-1-alpha, organism-specific biosystem
• Function: protein binding; ubiquitin protein ligase binding