Recombinant Human CUL2/RBX1 was coexpressed in Insect cells.
50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
E3 Ligase Assay: The activity of Cul2/Rbx1 was validated indirectly through its ability to act as a substrate for Neddylation in the presence of the NEDD8 E3 ligase His-DCNL2 and thioester-loaded His-UBE2M-NEDD8. Incubation of Cul2/Rbx1 and thioester loaded His-UBE2M-NEDD8 in the presence or absence of His-DCNL2 at 4˚C was compared at two time points T0 and T2 minutes. Neddylation of the Cul2 subunit in the presence of His-DCNL2 was demonstrated.
>75% by InstantBlue™ SDS-PAGE
12 months at -70 centigrade; aliquot as required
Store product at –70 centigrade