Active Recombinant Rat Prkaa1(T172D), GST/MYC-tagged

• Cat.No.: Prkaa1-144R
• Product Overview: Recombinant Rat Prkaa1 (amino acid residues 3-308), with N-terminal GST and N-terminal MYC, was expressed in E.coli. AMPK alpha 1 has a T172D mutation to mimic the activation of the enzyme through phosphorylation of Thr172 by PDK1.

Specification

• Description: The deubiquitylating enzyme (DUB) found to remove these ubiquitin chains from both NUAK1 and MARK4 has been identified as USP9X. AMPK activation has also been shown to increase the expression of the E3 ubiquitin ligases MAFBx/Atrogin-1 and MuRF1.
• Source: E. coli
• Species: Rat
• Form: 50 mM Tris/HCl pH7.5, 0.1 mM EGTA, 150 mM NaCl, 0.1% ?-Mercaptoethanol, 270 mM sucrose, 0.03% Brij-35, 1 mM Benzamidine, 0.2 mM PMSF.
• Bio-activity: 41.73 Units/mg (64.27 Units/ml)Substrate: AMARA peptide (AMARAASAAALARRR)
• Molecular Mass: ~64.6 kDa
• Purity: >95% by InstantBlue™ SDS-PAGE
• Unit Definition: 1 Unit = 1 nmole of phosphate incorporated into the substrate in 1 minute.
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 2.0 mg/ml

Gene Information

• Gene Name: Prkaa1 protein kinase, AMP-activated, alpha 1 catalytic subunit [ Rattus norvegicus ]
• Official Symbol: Prkaa1
• Synonyms: PRKAA1; protein kinase, AMP-activated, alpha 1 catalytic subunit; 5-AMP-activated protein kinase catalytic subunit alpha-1; AMPK alpha-1 chain; AMPK subunit alpha-1; tau-protein kinase PRKAA1; 5-AMP-activated protein kinase alpha-1 catalytic subunit; AMPKalpha1
• Gene ID: 65248
• mRNA Refseq: NM_019142
• Protein Refseq: NP_062015
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Energy dependent regulation of mTOR by LKB1-AMPK, organism-specific biosystem; Hypertrophic cardiomyopathy (HCM), organism-specific biosystem; Hypertrophic cardiomyopathy (HCM), conserved biosystem; IRS-mediated signalling, organism-specific biosystem; IRS-related events, organism-specific biosystem
• Function: AMP-activated protein kinase activity; AMP-activated protein kinase activity; AMP-activated protein kinase activity; ATP binding; chromatin binding; chromatin binding; eukaryotic elongation factor-2 kinase activator activity; histone serine kinase activity; histone serine kinase activity; kinase binding; metal ion binding; nucleotide binding; protein C-terminus binding; protein binding; protein serine/threonine kinase activity; tau-protein kinase activity