Active Recombinant Human TNFAIP3, GST-tagged
Cat.No. : | TNFAIP3-156H |
Product Overview : | Recombinant human TNFAIP3 (amino acid residues 1-336), fused with N-terminal GST, was expressed in E. coli. |
- Specification
- Gene Information
- Related Products
Description : | There are two main classes of DUB, cysteine proteases and metalloproteases. A20 is a cysteine protease and is a member of the OTU (ovarian tumour) superfamily of proteins (Balakirev et al., 2003). Cloning of the human gene was first described by Opipari et al. (1990). A20, or TNFAIP3, is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitylase activities. The N-terminal domain of A20 functions as a deubiquitylating enzyme by removing K63-linked ubiquitin chains from receptor-interacting protein (RIP) essential mediator of the proximal TNF receptor-1 (TNFR1) signalling complex. |
Source : | E. coli |
Species : | Human |
Tag : | GST |
Form : | 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol |
Bio-activity : | Deubiquitylase Enzyme Assay: The activity of GST-A20 CD(1-366) was validated by the monitoring of mono-ubiquitin generation as a result of the enzyme catalysed cleavage of K48-linked di-ubiquitin. Incubation of the substrate in the presence or absence of GST-A20 CD(1-366) was compared confirming the deubiquitylating activity of GST-A20 CD(1-366). |
Molecular Mass : | ~70.9kDa |
Purity : | >98% by SDS-PAGE |
Storage : | 12 months at -70°C. Avoid multiple freeze/thaw cycles. |
Concentration : | 0.5 mg/ml |
Gene Name : | TNFAIP3 tumor necrosis factor, alpha-induced protein 3 [ Homo sapiens ] |
Official Symbol : | TNFAIP3 |
Synonyms : | TNFAIP3; tumor necrosis factor, alpha-induced protein 3; tumor necrosis factor alpha-induced protein 3; A20; OTUD7C; TNFAIP3 (A20); zinc finger protein A20; TNF alpha-induced protein 3; OTU domain-containing protein 7C; putative DNA-binding protein A20; tumor necrosis factor inducible protein A20; TNFA1P2; MGC104522; MGC138687; MGC138688; |
Gene ID : | 7128 |
mRNA Refseq : | NM_006290 |
Protein Refseq : | NP_006281 |
MIM : | 191163 |
UniProt ID : | P21580 |
Chromosome Location : | 6q23-q25 |
Pathway : | CD40/CD40L signaling, organism-specific biosystem; Canonical NF-kappaB pathway, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Measles, organism-specific biosystem; Measles, conserved biosystem; NOD-like receptor signaling pathway, organism-specific biosystem; |
Function : | DNA binding; cysteine-type endopeptidase inhibitor activity involved in apoptotic process; cysteine-type peptidase activity; kinase binding; ligase activity; metal ion binding; peptidase activity; protease binding; protein binding; protein self-association; ubiquitin binding; ubiquitin thiolesterase activity; ubiquitin-protein ligase activity; ubiquitin-specific protease activity; ubiquitin-specific protease activity; zinc ion binding; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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