Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Active Recombinant Human UBA7, His-tagged

Cat.No. : UBA7-171H
Product Overview : Recombinant human UBA7 (amino acid residues 1-1012), fused with N-terminal His, was expressed in Insect sf21.
  • Specification
  • Gene Information
  • Related Products
Description : UBA7 is a member of the E1 activating enzyme family and cloning of the human gene was first described by Kok et al. (1993). The UBA7 gene has been mapped to chromosome 3p21 by high resolution fluorescence in situ hybridization (Carritt et al. 1992). UBA7 and UBE2L6 have been reported to function as the E1 and E2 enzymes respectively for ISG conjugation forming a thioester intermediate through Cys-131 of UBE2L6 (Takeuchi et al. 2005).
Source : Insect cells
Species : Human
Tag : His
Form : 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity : E1 Thioester ISG15 Loading Assay: The activity of His-UBA7 was validated by loading ISG15 onto the active cysteine of His-UBA7. Incubation of the His-UBA7 enzyme in the presence of ISG15 and ATP at 30?C was compared at two time points, T0 and T10 minutes. Sensitivity of the ISG15/His-UBA7 thioester bond to the reducing agent DTT was confirmed.
Molecular Mass : 116.53 kDa
Purity : >98% by SDS-PAGE
Storage : 12 months at -70°C. Avoid multiple freeze/thaw cycles.
Concentration : 0.5 mg/ml
Gene Name : UBA7 ubiquitin-like modifier activating enzyme 7 [ Homo sapiens ]
Official Symbol : UBA7
Synonyms : UBA7; ubiquitin-like modifier activating enzyme 7; UBE1L, ubiquitin activating enzyme E1 like; ubiquitin-like modifier-activating enzyme 7; D8; UBA1; ubiquitin activating enzyme E1 homolog B (yeast); UBA1B; ubiquitin activating enzyme E1; UBE2; ubiquitin-activating enzyme 7; ubiquitin-activating enzyme-2; UBA7, ubiquitin-activating enzyme E1; ubiquitin-activating enzyme E1 homolog; UBA1, ubiquitin-activating enzyme E1 homolog B; ubiquitin-activating enzyme E1-related protein; UBE1L; MGC12713;
Gene ID : 7318
mRNA Refseq : NM_003335
Protein Refseq : NP_003326
MIM : 191325
UniProt ID : P41226
Chromosome Location : 3p21
Pathway : Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem;
Function : ATP binding; ISG15 activating enzyme activity; ligase activity; nucleotide binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (0)

Ask a question

Customer Reviews (0)

Write a review

Ask a Question for All UBA7 Products

Required fields are marked with *

My Review for All UBA7 Products

Required fields are marked with *

0

Inquiry Basket

cartIcon
logo

FOLLOW US

Terms and Conditions        Privacy Policy

Copyright © 2024 Creative BioMart. All Rights Reserved.

Contact Us

  • /

Stay Updated on the Latest Bioscience Trends