Active Recombinant Human UCHL5, GST-tagged

• Cat.No.: UCHL5-181H
• Product Overview: Recombinant human UCHL5 (amino acid residues 1-328), fused with N-terminal GST, was expressed in E.coli.

Specification

• Description: The deubiquitylating activity of the proteasome has been attributed to the action of three deubiquitylases: UCHL5, USP14 and RPN11, which are all localised in the 19S regulatory particle. It has been reported that loss of both UCHL5 and USP14 leads to the accumulation of polyubiquitylated proteins and an inhibition of protein degradation without altering the structure or catalytic properties of the proteasome (D"Arcy et al., 2011).
• Source: E. coli
• Species: Human
• Tag: GST
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: Deubiquitylase Enzyme Assay: The activity of GST-UCHL5 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-UCHL5 was compared confirming the deubiquitylating activity of GST-UCHL5.
• Molecular Mass: ~64 kDa
• Purity: >88% by SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: UCHL5 ubiquitin carboxyl-terminal hydrolase L5 [ Homo sapiens ]
• Official Symbol: UCHL5
• Synonyms: UCHL5; ubiquitin carboxyl-terminal hydrolase L5; ubiquitin carboxyl-terminal hydrolase isozyme L5; CGI 70; INO80 complex subunit R; INO80R; UCH37; ubiquitin thioesterase L5; ubiquitin C-terminal hydrolase UCH37; ubiquitin carboxyl-terminal esterase L5; CGI-70; UCH-L5
• Gene ID: 51377
• mRNA Refseq: NM_001199261
• Protein Refseq: NP_001186190
• MIM: 610667
• UniProt ID: Q9Y5K5
• Chromosome Location: 1q32
• Function: cysteine-type peptidase activity; endopeptidase inhibitor activity; omega peptidase activity; peptidase activity; proteasome binding; protein binding; ubiquitin thiolesterase activity; ubiquitin-specific protease activity