Active Recombinant Human AKR1B1 protein

• Cat.No.: AKR1B1-136H
• Product Overview: Recombinant Human AKR1B1(1-316aa) was expressed in E. coli.

Specification

• Description: Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.
• Source: E. coli
• Species: Human
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
• Bio-activity: Specific activity: approximately 0.2-0.9 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH/min at 25 centigrade. Specific activity was expressed as units/mg protein.
• Molecular Mass: 35.8kDa (316aa), confirmed by MALDI-TOF.
• AA Sequence: MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVKGACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKPAVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERIAENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF
• Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: AKR1B1 aldo-keto reductase family 1, member B1 (aldose reductase) [ Homo sapiens ]
• Official Symbol: AKR1B1
• Synonyms: AKR1B1; aldo-keto reductase family 1, member B1 (aldose reductase); ALDR1; aldose reductase; AR; aldehyde reductase 1; low Km aldose reductase; Lii5-2 CTCL tumor antigen; aldo-keto reductase family 1 member B1; ADR; ALR2; MGC1804
• Gene ID: 231
• mRNA Refseq: NM_001628
• Protein Refseq: NP_001619
• MIM: 103880
• UniProt ID: P15121
• Chromosome Location: 7q35
• Pathway: Fructose and mannose metabolism, organism-specific biosystem; Fructose and mannose metabolism, conserved biosystem; Galactose metabolism, organism-specific biosystem; Galactose metabolism, conserved biosystem; Glycerolipid metabolism, organism-specific biosystem; Glycerolipid metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem
• Function: alditol:NADP+ 1-oxidoreductase activity; aldo-keto reductase (NADP) activity; electron carrier activity; glyceraldehyde oxidoreductase activity; oxidoreductase activity