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Recombinant Human TOP2B, His-tagged

Cat.No. : TOP2B-19H
Product Overview : Recombinant hTOP2βcore (residues 445-1201), fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Theoretical pI: 6.5
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Description : This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants; however, the second variant has not yet been fully described.
Source : E.coli
Species : Human
Tag : His
Form : Liquid. 20 mM Tris-HCl, 200 mM NaCl, pH7.4.
Molecular Mass : ~87 kDa
AA Sequence : SVKYSKIKGIPKLDDANDAGGKHSL ECTLILTEGDSAKSLAVSGLGVIGR DRYGVFPLRGKILNVREASHKQIME NAEINNIIKIVGLQYKKSYDDAESL KTLRYGKIMIMTDQDQDGSHIKGLL INFIHHNWPSLLKHGFLEEFITPIV KASKNKQELSFYSIPEFDEWKKHIE NQKAWKIKYYKGLGTSTAKEAKEYF ADMERHRILFRYAGPEDDAAITLAF SKKKIDDRKEWLTNFMEDRRQRRLH GLPEQFLYGTATKHLTYNDFINKEL ILFSNSDNERSIPSLVDGFKPGQRK VLFTCFKRNDKREVKVAQLAGSVAE MSAYHHGEQALMMTIVNLAQNFVGS NNINLLQPIGQFGTRLHGGKDAASP RYIFTMLSTLARLLFPAVDDNLLKF LYDDNQRVEPEWYIPIIPMVLINGA EGIGTGWACKLPNYDAREIVNNVRR MLDGLDPHPMLPNYKNFKGTIQELG QNQYAVSGEIFVVDRNTVEITELPV RTWTQVYKEQVLEPMLNGTDKTPAL ISDYKEYHTDTTVKFVVKMTEEKLA QAEAAGLHKVFKLQTTLTCNSMVLF DHMGCLKKYETVQDILKEFFDLRLS YYGLRKEWLVGMLGAESTKLNNQAR FILEKIQGKITIENRSKKDLIQMLV QRGYESDPVKAWKEAQEKAAEEDET QNQHDDSSSDSGTPSGPDFNYILNM SLWSLTKEKVEELIKQRDAKGREVN DLKRKSPSDLWKEDLAAFVEELDKV ESQERED
Purity : > 92% as determined by SDS-PAGE
Storage : Short Term Storage at +4°C, Long Term Storage at -20°C to -80°C. Avoid freeze/thaw cycles.
Gene Name : TOP2B topoisomerase (DNA) II beta 180kDa [ Homo sapiens (human) ]
Official Symbol : TOP2B
Synonyms : TOPIIB; top2beta; TOP2B; DNA topoisomerase II, beta isozyme; topo II beta; antigen MLAA-44; topoisomerase IIb; topoisomerase II beta; U937 associated antigen; DNA topoisomerase II beta; DNA topoisomerase II, 180 Kd; EC 5.99.1.3; DNA topoisomerase 2-beta
Gene ID : 7155
mRNA Refseq : NM_001068
Protein Refseq : NP_001059
MIM : 126431
UniProt ID : Q02880
Chromosome Location : 3p24
Function : ATP binding; DNA topoisomerase (ATP-hydrolyzing) activity; chromatin binding; histone deacetylase binding; nucleotide binding; protein C-terminus binding; protein heterodimerization activity; protein kinase C binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What cellular processes does TOP2B participate in, besides replication? 01/14/2022

TOP2B is crucial for chromosome segregation, transcription, and DNA recombination, playing a central role in maintaining genomic stability.

How does TOP2B contribute to the regulation of chromatin structure? 06/18/2021

TOP2B helps in remodeling chromatin by resolving supercoils, influencing the accessibility of DNA for various cellular processes.

What is the relationship between TOP2B and drug resistance in cancer treatment? 04/07/2020

Increased expression of TOP2B has been associated with resistance to certain chemotherapeutic agents, impacting the efficacy of cancer treatments.

What is the consequence of TOP2B mutations on cellular function? 08/22/2019

Mutations in TOP2B can lead to impaired DNA repair, genomic instability, and increased susceptibility to various diseases, including certain types of cancer.

How is TOP2B expression regulated in cells? 11/03/2018

Various factors, including cell cycle progression and response to DNA damage, influence the expression of TOP2B to meet the dynamic needs of the cell.

Customer Reviews (3)

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01/08/2022

    The quality of this product exceeded my expectations, providing precise and reliable results in our experiments.

    09/25/2019

      Rapid delivery! Ordered and received the product within a short timeframe, allowing us to proceed with our experiments without delays.

      04/18/2018

        Exceptional service!

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