Active Recombinant Mouse Il13

• Cat.No.: Il13-16M
• Product Overview: A DNA sequence encoding the mouse IL13 (Pro22-Phe131) was expressed and purified.

Specification

• Description: Interleukin 13 (IL-13) is a single-chain glycosylated polypeptide, which belongs to the IL-13/IL-4 family. IL-13 protein is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 exerts its effects through a multi-subunit receptor comprising the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains (IL-13 Rα1 and IL-13 Rα2). As a cytokine, IL-13 protein is critical in regulating inflammatory, immune responses and diseases. In addition, it inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. IL-13 protein and antibody is more importantly implicated as a central mediator of immunoregulatory processes in various cell types.
• Source: Human Cells
• Species: Mouse
• Predicted N Terminal: Pro 22
• Form: Lyophilized from sterile PBS, pH 7.5
• Bio-activity: Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50 for this effect is typically 2-10 ng/mL.
• Molecular Mass: The recombinant mouse IL13 consists of 110 amino acids and predicts a molecular mass of 12.1 KDa. It migrates as an approximately 14 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70°C
• Storage: Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Publication:
  IFN-γ Limits Immunopathogenesis but Delays Fungal Clearance during Pneumocystis Pneumonia (2023)

Gene Information

• Gene Name: Il13 interleukin 13 [ Mus musculus (house mouse) ]
• Official Symbol: Il13
• Synonyms: Il13; interleukin13; Il-13;interleukin-13;OTTMUSP00000005816; T-cell activation protein P600; IL-13
• Gene ID: 16163
• mRNA Refseq: NM_008355
• Protein Refseq: NP_032381
• UniProt ID: P20109
• Chromosome Location: 11 B1.3; 11 31.98 cM
• Pathway: Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Glucocorticoid receptor regulatory network
• Function: cytokine activity; interleukin-13 receptor binding; cytokine receptor binding