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Human Esophagus Tumor Lysate

Cat.No. : Esophagus-1H
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Species : Human
Concentration : 2 mg/ml
Preparation method : Tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 g/ml of aprotinin, 5 g/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% -mercaptoethanol.
Recommended Usage : For research use only, not for diagnostic or therapeutic use.
Storage Buffer : Lysate is supplied in SDS sample buffer containing 5% -mercaptoethanol
Applications : Cell/tissue Protein Lysates. Western blot-Load 5-20 ug/lane for testing with various antibodies. Heat at 95oC for 2-5 minutes prior to loading on gels.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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