Recombinant E.coli DnaK Chaperone Hsp70, Co-chaperone with DnaJ (508-638)
Cat.No. : | DnaK-316E |
Product Overview : | Recombinant DNAK was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques (132 aa, 14.6 kDa). Additional amino acid(Met) is attached at N- terminus. |
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Cat. No. : | DnaK-316 |
Description : | DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. Dnak(residues 508-638) of the substrate binding domain is a-helical and appears to act as a lid covering the substrate binding cleft. |
Source : | E.coli. |
Formulation : | Liquid. In 25 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 1 mM DTT, 10% glycerol. |
Molecular Weight : | 14.6 kDa (132aa). |
Purity : | > 95% by SDS – PAGE. |
Concentration : | 1 mg/ml (determined by Bradford assay). |
Sequence : | MNEDEIQKMV RDAEANAEAD RKFEELVQTR NQGDHLLHST RKQVEEAGDK LPADDKTAIE SALTALETAL KGEDKAAIEA KMQELAQVSQ KLMEIAQQQH AQQQTAGADA SANNAKDDDV VDAEFEEVKD KK |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Gene Name : | dnaK |
Synonyms : | dnaK; dnaK chaperone Hsp70, co-chaperone with DnaJ; Hsp70 protein; ECK0014; groPAB; groPC; groPF; grpC; grpF; JW0013; seg; chaperone Hsp70; DNA biosynthesis; autoregulated heat shock proteins. |
Gene ID : | 944750 |
Protein Refseq : | NP_414555.1 |
UniProt ID : | P0A6Y8 |
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