Recombinant Human Heat Shock 70kDa Protein 9 (Mortalin), His-tagged

• Cat.No.: HSPA9-334H
• Product Overview: Recombinant human HSPA9 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (654 aa, 71 kDa).

Specification

• Cat. No.: HSPA9-334H
• Description: HSPA9 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. HSPA9 was localized to chromosome 5, band q31, a region that is frequently deleted in myeloid leukemias and myelodysplasia (MDS), making it a candidate tumor suppressor gene, which is consistent with the biological function of its murine homologue. Also it inhibits nuclear translocation, transcriptional activation, and control of centrosome-duplication functions of p53.
• Source: E.coli.
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 0.5mM DTT, 10% glycerol.
• Molecular Weight: 71 kDa (654 aa).
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequence: MGSSHHHHHH SSGLVPRGSH MASEAIKGAV VGIDLGTTNS CVAVMEGKQA KVLENAEGAR TTPSVVAFTA DGERLVGMPA KRQAVTNPNN TFYATKRLIG RRYDDPEVQK DIKNVPFKIV RASNGDAWVE AHGKLYSPSQ IGAFVLMKMK ETAENYLGHT AKNAVITVPA YFNDSQRQAT KDAGQISGLN VLRVINEPTA AALAYGLDKS EDKVIAVYDL GGGTFDISIL EIQKGVFEVK STNGDTFLGG EDFDQALLRH IVKEFKRETG VDLTKDNMAL QRVREAAEKA KCELSSSVQT DINLPYLTMD SSGPKHLNMK LTRAQFEGIV TDLIRRTIAP CQKAMQDAEV SKSDIGEVIL VGGMTRMPKV QQTVQDLFGR APSKAVNPDE AVAIGAAIQG GVLAGDVTDV LLLDVTPLSL GIETLGGVFT KLINRNTTIP TKKSQVFSTA ADGQTQVEIK VCQGEREMAG DNKLLGQFTL IGIPPAPRGV PQIEVTFDID ANGIVHVSAK DKGTGREQQI VIQSSGGLSK DDIENMVKNA EKYAEEDRRK KERVEAVNMA EGIIHDTETK MEEFKDQLPA DECNKLKEEI SKMRELLARK DSETGENIRQ AASSLQQASL KLFEMAYKKM ASEREGSGSS GTGEQKEDQK EEKQ
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: HSPA9
• Synonyms: HSPA9; heat shock 70kDa protein 9 (mortalin); CSA; MOT; MOT2; GRP75; PBP74; mot-2; HSPA9B; MGC4500; MTHSP75; HSPA9; Mortalin; mortalin-2; mthsp75; p66-mortalin; 75 kDa glucose regulated protein; 75 kDa glucose-regulated protein; GRP 75; Heat shock 70 kDa protein 9; Peptide-binding protein 74; heat shock 70kD protein 9; heat shock 70kD protein 9B; heat shock 70kDa protein 9; heat shock 70kDa protein 9 (mortalin); heat shock 70kDa protein 9B (mortalin-2); mortalin, perinuclear; stress-70 protein, mitochondrial; peptide-binding protein 74.
• Gene ID: 3313
• mRNA Refseq: NM_004134.5
• Protein Refseq: NP_004125.3
• MIM: 600548
• UniProt ID: P38646
• Chromosome Location: 5q31.1
• Function: ATP binding; nucleotide binding;unfolded protein binding.