| Cat. No. : |
PPIH-111H |
| Description : |
Cyclophilin H (also known as peptidylpropyl isomerase H, PPIH) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-transisomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. The cyclophilin H is a specific component of the human U4/U6 small nuclear ribonucleoprotein particle involved in the nuclear splicing of premRNA.It stably associates with the U4/U6-60kD and -90kD proteins, the human orthologues of the Saccharomyces cerevisiaePrp4 and Prp3 splicing factors. |
| Sequences of amino acids : |
MAVANSSPVN PVVFFDVSIGG QEVGRMKIEL FADVVPKTAE NFRQFCTGEF RKDGVPIGYK GSTFHRVIKD FMIQGGDFVN GDGTGVASIY RGPFADENFK LRHSAPGLLS MANSGPSTN GCQFFITCSK CDWLDGKHV VFGKIIDGLL VMRKIENVPT GPNNKPKLPV V ISQCGEM |
| Formulation : |
Liquid in phosphate-buffered saline ( pH 7.4) containing 10% glycerol. |
| Purity : |
> 95% by SDS PAGE |
| Concentration : |
1 mg/ml (determined by Bradford assay) |
| Biological activity : |
Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin. |
| Activity Assay : |
1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.2. Add 10ul of recombinant Cyclophikin H (PPIH) protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)5. Record the increase in A405 nm for 30 minutes at 25°C. |
| Storage : |
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
