Species : |
Human |
Form : |
Sterile filtered white lyophilized (freeze-dried) powder. Recombinant Human Insulin was lyophilized from a concentrated (1 mg/mL) solution with no additives. Recombinant Human Insulin is purified by proprietary chromatographic techniques. Less than 1% dimers and aggregates as determined by silver-stained SDS-PAGE gel analysis. |
Bio-activity : |
Recombinant Human Insulin is fully biologically active when compared to WHO reference standard which is 28 units/mg. |
Endotoxin : |
Less than 0.1 ng/μg (IEU/μg) of Recombinant Human Insulin. |
Purity : |
Greater than 98.0% as determined by: a. Analysis by RP-HPLC b. Anion-exchange FPLC c. Analysis by reducing and non-reducing SDS-Page Silver-Stained gel. Protein content was determined by two independent methods: UV spectroscopy at 280 nm and Analysis by RP-HPLC, using a calibrated solution of Insulin as a reference standard. |
Usage : |
For research use only. Not for use in diagnostics or in humans. |
Storage : |
Recombinant Human Insulin, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, Recombinant Human Insulin should be stored at 4°C for between 2 – 7 days and below -18°C for storage longer than 7 days. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid freeze-thaw cycles. |
Warning : |
No warranties, expressed or implied, are made regarding the use of this product. Creative Biomart is not liable for any damage, personal injury, or economic loss caused by this product. |