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Recombinant Mouse TNF-alpha Soluble, FLAG-tagged

Cat.No. : TNF-64M
Product Overview : The extracellular domain of mouse tumor necrosis factor-α (TNF-α) (amino acids 77-235) is fused at the N-terminus to an 8 amino acid linker peptide and a FLAG tag.
  • Specification
  • Gene Information
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Species : Mouse
Tag : FLAG
Form : Lyophilized powder containing 50 μg recombinant TNF-α and PBS, 1 mg/mL TNF-α after reconstitution.
Molecular Mass : Molecular weight ~20 kDa by SDS-PAGE.
Endotoxin : < 0.1 eu/μg purified protein (lal test; bio>Whittaker).
Purity : ≥ 95% as determined by SDS-PAGE.
Characteristic : ED50: 0.02 ng/mL (WEHI 164 cells). TNF-α binds to human, mouse and rat TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer, TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane bound TNF-α.
Applications : Induction of TNF-R1 and TNF-R2 mediated responses. Induction of TNF-R2 mediated responses requires addition of a secondary cross-linking anti-FLAG antibody.Inducing Apoptosis: Recombinant mouse TNF-α protein induces apoptosis in WEHI 164 and other TNF-α sensitive cells. Optimal concentration varies with cell type and should be determined by testing serial dilutions on cells (For WEHI 164 cells use 0.5-1 ng/mL). The optimal dilution for a specific application should be determined by the researcher.
Usage : For in vitro research use only. Not for use in diagnostics or in humans.
Storage : Store at -20°C. Aliquot solutions to avoid repeated freeze/thaw. Lyophilized product is stable at -20°C for at least 6 months.
Reconstitution : Prepare a 1 mg/mL stock solution by dissolving the contents of the vial in 50 μL of sterile H20. Further dilutions should be made with medium containing 5% fetal calf serum.
Warning : No warranties, expressed or implied, are made regarding the use of this product. Creative Biomart is not liable for any damage, personal injury, or economic loss caused by this product.
Gene Name : Tnf tumor necrosis factor [ Mus musculus ]
Official Symbol : TNF
Synonyms : TNF; tumor necrosis factor; TNF-a; TNF alpha; cachectin; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; MGC151434;
Gene ID : 21926
mRNA Refseq : NM_013693
Protein Refseq : NP_038721
Pathway : Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem;
Function : cytokine activity; cytokine activity; identical protein binding; protease binding; protease binding; protein binding; transcription regulatory region DNA binding; tumor necrosis factor receptor binding; tumor necrosis factor receptor binding; tumor necrosis factor receptor binding; tumor necrosis factor receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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