Active Bovine Fibronectin, carrier free

• Cat.No.: FN1-3B
• Product Overview: Purified Natural Bovine Fibronectin is purified from bovine plasma by affinity chromatography on gelatin-Sepharose.

Specification

• Description: Fibronectin is a high-molecular weight (~440kDa) extracellular matrix glycoprotein that binds to membrane-spanning receptor proteins called integrins.[1] In addition to integrins, fibronectin also binds extracellular matrix components such as collagen, fibrin and heparan sulfate proteoglycans (e.g. syndecans). Fibronectin exists as a dimer, consisting of two nearly identical monomers linked by a pair of disulfide bonds. The fibronectin protein is produced from a single gene, but alternative splicing of its pre-mRNA leads to the creation of several isoforms.
• Source: bovine plasma
• Species: Bovine
• Form: Supplied as a 0.2 μm filtered solution in 0.4 M Urea, 50 mM Tris-HCl (pH 7.5) and 0.5 M NaCl, at a concentration of 1.36 mg/mL.
• Bio-activity: Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. In this application, the recommended concentration for this effect is typically 1 - 5 μg/cm2. Fibronectin can also be added in the media to support cell spreading at a concentration of 0.5 - 50 μg/mL.
• Endotoxin: <1.0 eu per 1 μg of the protein by the lal</1.0
• Purity: >90%, as determined by SDS-PAGE and visualized by silver stain.
• Applications: Adherent Cell Assays
• Usage: FOR RESEARCH USE ONLY
• Quality Control Test: Samples are stable for up to six months at 2 - 8 centigrade. Avoid vortexing and excessive agitation. Aliquot into 50 μL to 100 μL volumes and store at ≤ -20 centigrade for up to six months in a manual defrost freezer.
• Concentration: 1.36 mg/mL
• Warning: Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: FN1 fibronectin 1 [ Bos taurus ]
• Official Symbol: FN1
• Synonyms: FN; fibronectin; fibronectin ED-A- region; fibronectin variable region; embryo-specific fibronectin 1 transcript; cumulus cell-specific fibronectin 1 transcript
• Gene ID: 280794
• mRNA Refseq: NM_001163778
• Protein Refseq: NP_001157250
• Chromosome Location: chromosome: 2
• Pathway: Amoebiasis, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem
• Function: heparin binding; peptidase activator activity