Recombinant Human NOTCH1 protein, Fc-tagged
Recombinant Human NOTCH1(Ala19 - Gln526) fused with Fc of Human IgG1 at C-terminal was expressed in NS0.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by its binding ability in a functional ELISA. Immobilized rhNotch-1 Fc Chimera at 5 µg/mL (100 µL/well) can bind rrJagged-1/Fc Chimera with an apparent KD <5 nM in a functional ELISA.
Predicted Molecular Mass: 80.1 (monomer) kDa;SDS-PAGE: 115-125 kDa, reducing conditions.
<1.0 EU per 1 μg of the protein by the LAL method.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in sterile PBS.