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Recombinant Streptomyces Avidinii Streptavidin, His-tagged

Cat.No. : Streptavidin-390S
Product Overview : Recombinant His tagged streptavidin was expressed in E.coli and purified by using conventional chromatography techniques,17 kDa (167 aa).
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Cat. No. : Streptavidin-390S
Description : Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
Sequences of amino acids : MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ
Formulation : Liquid. In 20 mM Tris-HCl buffer (pH 7.5)
Purity : > 95% by SDS - PAGE
Concentration : 1 mg/ml (determined by Bradford assay)
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does Streptavidin differ from natural streptavidin in its formulation? 01/23/2022

Streptavidin formulations often include shortened variants (core streptavidin proteins) with shortened N and C termini compared to natural streptavidin. These variants offer improved characteristics in terms of homogeneity, solubility, resistance to protein hydrolysis, and accessibility of the biotin-binding pocket.

What is Streptavidin? 10/20/2021

Streptavidin is a 55kDa tetrameric protein secreted by Streptomyces avidinii, known for its strong binding affinity with biotin.

What is the binding partner of Streptavidin? 12/17/2020

Streptavidin tightly binds to biotin (sc-204706).

What is the dissociation constant (Kd) of the Biotin-Streptavidin complex? 01/22/2019

The dissociation constant (Kd) for the Biotin-Streptavidin complex is approximately ~10^15M, indicating an exceptionally strong binding affinity.

What are the key features of the Streptavidin/Biotin system? 03/18/2018

The Streptavidin/Biotin system exhibits one of the largest observed binding free energies, making it suitable for non-covalent binding of proteins and small ligands in aqueous solutions. The complex is also highly stable over a broad range of temperature and pH values.

What is the origin of Streptavidin? 03/05/2018

Streptavidin is produced by Streptomyces avidinii.

Why is Streptavidin widely used in molecular biology? 01/15/2017

Streptavidin is widely used in molecular biology due to its unique high affinity for biotin, making it a valuable tool for various applications.

Customer Reviews (3)

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Reviews
12/09/2022

    Its versatility in integrating with other laboratory reagents streamlined our workflow and improved efficiency.

    08/07/2020

      The product's customizability allowed us to fine-tune experiments to match our specific research objectives.

      03/13/2020

        Tailoring the product to our unique experimental conditions enhanced the precision of our results.

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