Recombinant E.coli RecA

• Cat.No.: recA-57E
• Product Overview: A recombinant E. coli strain overexpressing E. coli recA from a plasmid.

Specification

• Description: RecA functions in DNA recombination and DNA repair. RecA binds to single stranded DNA, resulting in the polymerization of RecA into a nucleoprotein complex. This complex can align with complementary double stranded DNA, resulting in RecA catalysis of DNA strand exchange. RecA DNA binding is stimulated by ATP hydrolysis or non-hydrolyzable ATP analogs. The RecA-ATP-single stranded DNA complex also can function as a coprotease factor in the proteolytic cleavage of LexA, UmuD and certain bacteriophage proteins. RecA complexed with site-specific oligonucleotides have been used to target and specifically cleave large DNA fragments.
• Source: E. coli
• Species: E. coli
• Molecular Mass: 37,973 Daltons
• Purity: >95% by SDS-PAGE
• Unit Definition: Sold by mass of pure protein determined at OD280 (A280 = 0.516 at 1 mg/mL, 1cm)..
• Storage: -25 to -15°C
• Concentration: 2.0 mg/mL
• Storage Buffer: Supplied in: 10mM Tris-HCl, 1mM DTT. 0.1 mM EDTA 50% glycerol pH 7.5 @ 25?C. Supplied with: 10X RecA Reaction Buffer: 700mM Tris-HCl, 100mM MgCl2, 50mM DTT pH 7.6 @ 25?C.

Gene Information

• Gene Name: recA?DNA recombination and repair protein; ssDNA-dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP-dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor [Escherichia coli str. K-12 substr. MG1655?]
• Official Symbol: recA
• Synonyms: recA; DNA recombination and repair protein; ssDNA-dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP-dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor; NP_417179.1; DNA strand exchange and renaturation, DNA-dependent ATPase, DNA- and ATP-dependent coprotease
• Gene ID: 947170
• Protein Refseq: NP_417179
• Pathway: Homologous recombination
• Function: peptidase activity