Recombinant Human Vascular Endothelial Growth Factor 121, His-tagged

• Cat.No.: VEGFA-130H
• Product Overview: Recombinant VEGF121 protein was expressed in E.coli and purified by using conventional chromatography techniques, 16.3 kDa (142aa).

Specification

• Cat. No.: VEGFA-130H
• Description: Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR
• Formulation: Liquid in 20mM Tris pH 8.0 containing 10% glycerol.
• Molecular Weight: 16.3 kDa (142aa), confirmed by MALDI-TOF
• Purity: > 95% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Endotoxin Level: The ED50 for this effect is< 4.2 ng/ml. measured in a cell proliferation assay using nih-3t3>
• Biological activity: The ED50 for this effect is< 4.2 ng/ml. measured in a cell proliferation assay using nih-3t3>
• Activity Assay: 1.Cell line: NIH-3T3 (Mus mosculus/ embryo) 2.Maintenance Condition: RPMI 1640 containing 10% FBS 3.Assay Medium: serum free RPMI 1640 4.Cell Density: 2 x 104 cells/well (96 well plate, final volume 100ul)5.Serum Free Starvation : 24hr with RPMI1640 6.Incubation Time: 24 hr (after sample treatment)7.Concentration Range: 100 pg/ml - 10 ug/ml 8.Detection method: BrdU assay
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens ]
• Synonyms: VEGFA; vascular endothelial growth factor A; VPF; VEGF; MVCD1; VEGF-A; MGC70609; vascular permeability factor; vascular endothelial growth factor isoform VEGF165; Vascular permeability factor; vascular endothelial growth factor A
• Gene ID: 7422
• mRNA Refseq: NM_001025366
• Protein Refseq: NP_001020537
• MIM: 192240
• UniProt ID: P15692
• Chromosome Location: 6p12
• Pathway: Cytokine-cytokine receptor interaction; Focal adhesion; Pancreatic cancer; Renal cell carcinoma; VEGF signaling pathway; mTOR signaling pathway; Hemostasis; Signaling by VEGF
• Function: cell surface binding;cytokine activity;extracellular matrix binding;fibronectin binding;growth factor activity;heparin binding;platelet-derived growth factor receptor binding;protein homodimerization activity;vascular endothelial growth factor receptor 1 binding;vascular endothelial growth factor receptor 2 binding