Recombinant Human Tyrosine 3-monooxygenase/ Tryptophan 5-monooxygenase Activation Protein, Beta Polypeptide

• Cat.No.: YWHAB-103H
• Product Overview: Recombinant human 14-3-3 beta was expressed in E.coli and purified by using conventional chromatography techniques (246 aa, 28 kDa).

Specification

• Cat. No.: YWHAB-103H
• Description: The 14-3-3 family plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ and η that have been identified in mammals. The 14-3-3 beta, a subtype of the 14-3-3 proteins, was found in B Cells, brain and liver etc. This 14-3-3 betahas been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery.
• Source: E.coli.
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM EDTA, 50 mM NaCl.
• Molecular Weight: 28 kDa (246 aa).
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequence: MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL ELLDKYLIPN ATQPESKVFY LKMKGDYFRY LSEVASGDNK QTTVSNSQQA YQEAFEISKK EMQPTHPIRL GLALNFSVFY YEILNSPEKA CSLAKTAFDE AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD AGEGEN
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: YWHAB
• Synonyms: YWHAB; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; HS1; GW128; KCIP-1; Protein 1054; OTTHUMP00000031075; OTTHUMP00000031076; 14-3-3 protein beta/alpha; Protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; brain protein 14-3-3, beta isoform; YWHAB tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide.
• Gene ID: 7529
• mRNA Refseq: NM_003404.3
• Protein Refseq: NP_003395.1
• MIM: 601289
• UniProt ID: P31946
• Chromosome Location: 20q13.1
• Pathway: Cell Cycle; Apoptosis; Signaling by EGFR; Signaling by Insulin receptor; Signalling by NGF.
• Function: enzyme binding; protein domain specific binding.