TargetLock Protein A Beads

• Cat.No.: Protein A-33A
• Product Overview: The origin of protein A is from the bacteria Staphylococcus aureus. Protein A is capable of binding most classes and subclasses of immunoglobulins from Goat, Rabbit, Mouse and Human. Our Protein A beads are produced using genetically engineered form of Protein A. Nonessential regions have been removed while leaving the immunoglobulins binding sites intact. The recombinant Protein A has been immobilized to Sepharose beads using proprietary TargetLock chemistry, in order to create a winner product for the affinity purification and immunoprecipitation of various mammalian immunoglobulins.

Specification

• Cat.no: Protein A-33A
• Matrix: Highly cross-linked spherical agarose, 4%
• Average particle size: 40‐165 μm
• Dynamic binding capacity: ~4-8 mg rabbit IgG per ml of resin
• Recommended flow rate: 1-3 ml/min/ cm2
• pH working range: 2-11
• Elution buffer: 0.1M glycine buffer adjusted pH to 2.8 by acetic acid