Active Recombinant Human CSF2

• Cat.No.: CSF2-22H
• Product Overview: Recombinant Human GM-CSF is a 14 to 36 kDa globular protein consisting of 128 amino acids, containing two intramolecular disulfide bonds and two N-linked glycosylation sites.

Specification

• Description: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
• Source: CHO Cells
• Species: Human
• Form: 0.22 um filtered solution in 20 mM TRIS*HCl buffer pH 7.2
• Bio-activity: ED50<1 ng/ml, The activity was determined by the dose dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line)
• Molecular Mass: 14-36 kDA, glycosylated
• AA Sequence: APARSPSPSTQPWEHVNAIQEARRL LNLSRDTAAEMNETVEVISEMFDLQ EPTCLQTRLELYKQGLRGSLTKLKG PLTMMASHYKQHCPPTPETSCATQI ITFESFKENLKDFLLVIPFDCWEPV QE
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, Determined by SDS-PAGE under reducing conditions and visualized by silver stain.

Gene Information

• Gene Name: CSF2 colony stimulating factor 2 (granulocyte-macrophage) [ Homo sapiens (human) ]
• Official Symbol: CSF2
• Synonyms: CSF2; colony stimulating factor 2 (granulocyte-macrophage); GMCSF; MGC131935; MGC138897; colony stimulating factor 2; molgramostin; sargramostim; granulocyte-macrophage colony stimulating factor; GM-CSF; Molgramostin; Sargramostim; Colony-stimulating factor
• Gene ID: 1437
• mRNA Refseq: NM_000758
• Protein Refseq: NP_000749
• MIM: 138960
• UniProt ID: P04141
• Chromosome Location: 5q31.1
• Pathway: Calcineurin-regulated NFAT-dependent transcription in lymphocytes; Cytokine Signaling in Immune system; Cytokines and Inflammatory Response
• Function: cytokine activity; granulocyte macrophage colony-stimulating factor receptor binding; growth factor activity