Active Recombinant Rat Csf1

• Cat.No.: Csf1-14R
• Product Overview: Recombinant Rat Csf1 was expressed in CHO.

Specification

• Description: Macrophage-Colony Stimulating Factor (M-CSF), also known as Colony Stimulating Factor-1 (CSF-1), can stimulate the survival, proliferation and differentiation of mononuclear phagocytes, in addition to the spreading and motility of macrophages. M-CSF is mainly produced by monocytes, macrophages, fibroblasts, and endothelial cells. M-CSF interaction with its receptor, c-fms, has been implicated in the growth, invasion, and metastasis of of several diseases, including breast and endometrial cancers.
• Source: CHO
• Species: Rat
• Form: White Freeze-dried Powder. Lyophilized after extensive dialysis against PBS.
• Bio-activity: ED50 < 2.5 ng/ml, measured in a cell proliferation assay using Murine M-NFS-60 cells, corresponding to a specific activity of > 4×10^5 units/mg.
• Molecular Mass: 32-40 kDa, observed by non-reducing SDS-PAGE.
• AA Sequence: EVSEHCSHMI GNGHLQILQQ LIDSQMETAC LIEYKFVDQEQLDDPVCYLK KAFVLVQVII EETMRFKDNT PNANATERLQELSMKLNSCF IKDYKEQNEA CVQTYKESPL RLLEKIKNFFNETKNFLEKD WNIFSKNCND SLAKCSSRDV VTKP
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 95% as analyzed by SDS-PAGE.
• Storage: Lyophilized recombinant Rat Macrophage-Colony Stimulating Factor (M-CSF) remains stable up to 6 months at -80 ºC from date of receipt. Upon reconstitution, rrM-CSF should be stable up to 1 week at 4 °C or up to 2 months at -20 ºC.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/ml.

Gene Information

• Gene Name: Csf1 colony stimulating factor 1 (macrophage) [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Csf1
• Synonyms: Csf1; colony stimulating factor 1 (macrophage); macrophage colony-stimulating factor 1; CSF-1; MCSF; NP_076471.3; EC 2.7.10.1
• Gene ID: 78965
• mRNA Refseq: NM_023981
• Protein Refseq: NP_076471
• UniProt ID: Q8JZQ0
• Chromosome Location: 2q34
• Pathway: Cytokine-cytokine receptor interaction; Cytokines and Inflammatory Response (BioCarta); Hematopoietic cell lineage
• Function: cytokine activity; growth factor activity; macrophage colony-stimulating factor receptor binding