Recombinant Mouse Tigit(Gly 26 - Thr 143 ) fused with His tag at C-terminal was expressed in HEK293.
Predicted N Terminal:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
Has a calculated MW of 14.8 kDa. The reducing (R) protein migrates as 20-25 kDa in SDS-PAGE .
Gly 26 - Thr 143
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).