Recombinant Rhesus PVR protein, His-tagged
Recombinant Rhesus PVR(Met 1-Asn 343) fused with His tag at C-terminal was expressed in HEK293.
CD155, commonly known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5), is a type I transmembrane single-span glycoprotein, and belongs to the nectins and nectin-like (Necl) subfamily. CD155 was originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV), an etiologic agent of the central nervous system disease poliomyelitis. The normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155 may assist in an efficient humoral immune response generated within the intestinal immune system. It has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhension, migration and NK-cell killing, and thus efficiently prime cell-mediated tumor-specific immunity.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.41. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.
Measured by its binding ability in a functional ELISA . Immobilized Rhesus CD155 at 2 μg/ml (100 μl/well) can bind human CD226 with a linear ranger of 1.28-32 ng/ml.
The recombinant rhesus PVR consists of 327 amino acids and has a calculated molecular mass of 35.8 kDa.
Met 1-Asn 343
< 1.0 EU per μg of the protein as determined by the LAL method
> 98 % as determined by SDS-PAGE
Samples are stable for up to twelve months from date of receipt at -70 centigrade
Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.