Recombinant Human VWF(Asp1498-Val1665) fused with Met/His tag at N-terminal was expressed in E. coli.
Predicted N Terminal:
Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Measured by its ability to be used as a protein substrate for ADAMTS13. >50% of rhvWF-A2 is cleaved as measured under the described conditions.
Predicted Molecular Mass: 20 kDa;SDS-PAGE: 18 kDa, reducing conditions.
<1.0 EU per 1 μg of the protein by the LAL method.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 centigrade as supplied.3 months, -20 to -70 centigrade under sterile conditions after opening.