Active Recombinant Human FLT3LG

• Cat.No.: FLT3LG-152H
• Product Overview: Recombinant Human FLT3LG (Accession # AAA17999.1) Thr27-Pro185 was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Source: Baculovirus Sf 21 Insect cells
• Species: Human
• Predicted N Terminal: Thr27
• Form: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with mouse Flt-3.The ED50 for this effect is typically 0.2-1 ng/mL.
• Molecular Mass: Recombinant Human FLT3LG has a calculated MW of 17.5 kDa. In SDS-PAGE migrates as 17-30 kDa, reducing conditions.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: FLT3LG fms-related tyrosine kinase 3 ligand [ Homo sapiens ]
• Official Symbol: FLT3LG
• Synonyms: FLT3LG; fms-related tyrosine kinase 3 ligand; flt3 ligand; FL; FLT3L
• Gene ID: 2323
• mRNA Refseq: NM_001204502
• Protein Refseq: NP_001191431
• MIM: 600007
• UniProt ID: P49771
• Chromosome Location: 19q13.3