Recombinant Human GLB1(Met1-Val677) fused with His tag at C-terminal was expressed in CHO.
Predicted N Terminal:
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl-beta -D-galactopyranoside. The specific activity is >1,800 pmol/min/μg.
Predicted Molecular Mass; 74 kDa;SDS-PAGE: 70-95 kDa, reducing conditions;
<1.0 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 centigrade as supplied.3 months, -20 to -70 centigrade under sterile conditions after opening.