Active Recombinant Human PODXL protein, His-tagged

• Cat.No.: PODXL-218H
• Product Overview: Recombinant Human PODXL(Ser23-Arg427) fused with a C-terminal Asp and 10-His tag was expressed in NS0.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ser23
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to inhibit the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. The ED50 for this effect is typically 0.08-0.4 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 43 (monomer) kDa;
  SDS-PAGE: 125-165 kDa, reducing conditions.
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining.
• Stability: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.

Gene Information

• Gene Name: PODXL podocalyxin-like [ Homo sapiens ]
• Official Symbol: PODXL
• Synonyms: PODXL; podocalyxin-like; podocalyxin; Gp200; PC; PCLP; GCTM-2 antigen; podocalyxin-like protein 1; PCLP-1; MGC138240
• Gene ID: 5420
• mRNA Refseq: NM_001018111
• Protein Refseq: NP_001018121
• MIM: 602632
• UniProt ID: O00592
• Chromosome Location: 7q32-q33