Active Recombinant Human VTN

• Cat.No.: VTN-295H
• Product Overview: Recombinant Human VTN (Accession # AAH05046.1) Asp20-Leu478 was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Predicted N Terminal: Asp20
• Form: Lyophilized from a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells.When 5 x 10^4 cells/well are added to Vitronectin coated plates (5 µg/mL with 100 µL/well), approximately >55% will adhere after 30 minutes at 37 °C. Optimal concentration depends on cell type as well as the application or research objectives..
• Molecular Mass: Recombinant Human VTN has a calculated MW of 52.3 kDa. In SDS-PAGE migrates as 70-80 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: VTN vitronectin [ Homo sapiens ]
• Official Symbol: VTN
• Synonyms: VTN; vitronectin; vitronectin (serum spreading factor, somatomedin B, complement S protein); complement S protein; serum spreading factor; somatomedin B; VN; epibolin; S-protein; complement S-protein; serum-spreading factor; V75; VNT
• Gene ID: 7448
• mRNA Refseq: NM_000638
• Protein Refseq: NP_000629
• MIM: 193190
• UniProt ID: P04004
• Chromosome Location: 17q11.2