Active Recombinant Mouse Mfap5, His-tagged

• Cat.No.: Mfap5-316M
• Product Overview: Recombinant Mouse Mfap5 (Accession # AAH25131) Gln29-Leu164, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Gln29 predicted, sequencing might be blocked, no result obtained
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of BUD-8 human fibroblast cells.When 2 x 10^4 cells/well are added to Recombinant Mouse MAGP-2/MFAP5 coated plates (10 µg/mL with 100 µL/well), approximately 45 - 70% will adhere after 1 hour at 37 °C. Optimal dilutions should be determinded by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Mfap5, His-tagged has a calculated MW of 16.5 kDa. In SDS-PAGE migrates as 22-30 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Mfap5 microfibrillar associated protein 5 [ Mus musculus ]
• Official Symbol: Mfap5
• Synonyms: MFAP5; microfibrillar associated protein 5; microfibrillar-associated protein 5; MFAP-5; microfibril-associated glycoprotein 2; MAGP-2
• Gene ID: 50530
• mRNA Refseq: NM_015776
• Protein Refseq: NP_056591